SDF-1及其受体CXCR4在结直肠癌组织中的表达及与PCNA的关系  

Expression of stromal cell derived factor-1,CXC chemokine receptor 4 and PCNA proteins in human colorectal carcinoma

作　　者：郭云娣[1] 李小宁[1] 李明[2] 何影娟[3] 

机构地区：[1]苏州卫生职业技术学院苏州检验医学生物技术重点实验室,江苏省苏州市215009 [2]苏州市立医院,江苏省苏州市215008 [3]苏州九龙医院,江苏省苏州市215021

出　　处：《世界华人消化杂志》2011年第18期1959-1962,共4页World Chinese Journal of Digestology

摘　　要：目的:研究结直肠癌组织中基质细胞衍生因子(SDF-1)及其受体(CXCR4)的表达与结直肠癌生物学行为以及与核增殖抗原(PCNA)间的关系,以探讨SDF-1/CXCR4的表达以及PCNA在结直肠癌侵袭、转移中的生物学意义.方法:在结直肠癌组织芯片上,应用免疫组织化学Envision法检测SDF-1/CXCR4和PCNA的表达水平,分析SDF-1、CXCR4和PCNA在结直肠癌的表达与患者淋巴结转移状态、肿瘤分化程度的关系及其相互关系.结果:SDF-1的阳性表达率为60.2%(77/128),CXCR4的阳性表达率为43.8%(56/128).结直肠癌组织SDF-1及CXCR4的阳性表达率均高于癌旁正常黏膜组织(P<0.01).PCNA的阳性表达率为44.5%(57/128).SDF-1/CXCR4和PCNA与患者淋巴结转移状态、肿瘤分化程度相关(均P<0.05),SDF-1/CXCR4与PCNA之间呈正相关(r=0.084,P=0.005;r=0.087,P=0.030).结论:同时检测结直肠癌组织中的SDF-1/CXCR4和PCNA的表达对判断肿瘤的恶性程度和估计预后有一定意义.AIM：To investigate the correlations of the expression of stromal cell derived factor-1（SDF-1）,CXC chemokine receptor 4（CXCR4）,and proliferating cell nuclear antigen（PCNA） with the clinicobiological parameters of human colorectal carcinoma.METHODS：The expression of SDF-1/CXCR4 and PCNA proteins in human colorectal carcinoma was measured by immunohistochemistry on tissue microarrays.The relationship of expression of SDF-1/CXCR4 and PCNA proteins with the clinicobiological parameters of human colorectal carcinoma was analyzed.RESULTS：The positive rates of SDF-1 and CXCR4 expression were significantly higher in colorectal carcinoma than in tumor-adjacent tissue（both P 0.01）.The expression of SDF-1/CXCR4 and PCNA was significantly correlated with lymph node metastasis and tumor differentiation in colorectal carcinoma.The expression of SDF-1/CXCR4 was positively correlated with that of PCNA in colorectal carcinoma（r = 0.084,0.087;P = 0.005,0.030）.CONCLUSION：SDF-1/CXCR4 and PCNA may be useful parameters for evaluation of metastasis and prognosis in patients with colorectal carcinoma.

关 键 词：结直肠肿瘤 基质细胞衍生因子1 C×C趋化因子受体 核增殖抗原 组织芯片片 

分 类 号：R735.3[医药卫生—肿瘤]