敲低U251细胞miR-221/222表达对其基因组表达的影响  

Influence of expression of miR-221/222 on expression of genome in glioblastoma U251 cells

作　　者：杨旸[1] 张春智[1] 杨卫东[1] 韩磊[1] 张安玲[1] 浦佩玉[1] 康春生[1] 

机构地区：[1]天津市神经病学研究所神经肿瘤实验室,天津医科大学总医院神经外科300052

出　　处：《中华神经外科杂志》2011年第7期705-709,共5页Chinese Journal of Neurosurgery

基　　金：教育部新世纪优秀人才支持计划资助项目（NCET-07-0615）;国家自然科学基金资助项目（30772231）

摘　　要：目的 采用基因表达谱芯片和生物信息学方法研究miR-221/222对胶质瘤U251细胞株信号通路的调控作用.方法 miR-221/222反义寡核苷酸瞬时转染人胶质瘤细胞株后提取总RNA进行基因表达谱检测,对差异表达基因行生物信息学分析,将核心转录因子进行实验验证.结果 敲低胶质瘤U251细胞中miR-221/222表达后,基因表达谱分析确定158个差异表达基因;生物信息学分析发现干扰素-α信号通路是受差异表达基因调节最显著的通路,其中STAT1和STAT2是干扰素-α通路的核心蛋白.结论 抑制miR--221/222基因簇表达可部分通过干扰素-α通路上调STAT1和STAT2 mRNA的翻译水平,并使U251细胞mRNA的表达谱发生改变.Objective To study the cell signaling regulation of miR - 221/222 in U251 cell line by microarray analysis and bioinformation method. Method As - miR -221/222 was transfected into U251 human glioma cell line, and the total RNA was extracted for texting gene expression profile. Differentially expressed genes were analyzed by bio - information method and the core transcriptional factors were identified by Western blot and immunofluorescence. Results After down - regulation of miR -221/222 in glioma cell lines, 158 differentially expressed genes were confirmed by microarray analysis. It was found that INF - α signaling pathway was the most significant one regulated by the differentially experssed genes. Moreover, STAT1 and STAT2 were core proteins in INF - α signaling pathway. It was revealed by Western blot and immunofluorescence that the expression of STAT1 and STAT2 were up - regulated in U251 cells after knocked -down miR - 221/222. Conclusions Knockdown of miR -221/222 would up - regulate STAT1 and STAT2 expression partially through IFN - α pathway and result in the change of mRNA expression profiles in U251 cell line.

关 键 词：MIR-221 MIR-222 神经胶质瘤 干扰素-α信号通路 

分 类 号：R739.4[医药卫生—肿瘤]