电刺激嗅球对脑室下区神经干细胞增殖、迁移和分化的影响  

Effects of electrical stimulation of olfactory bulb on proliferation, migration and differentiation of neural stem cells in subventricular zone

作　　者：张广慧[1] 秦新月[1] 王恬竹[1] 冯金洲[1] 郭振委[1] 

机构地区：[1]重庆医科大学附属第一医院神经内科、重庆市教委神经病学重点实验室,400016

出　　处：《中华神经外科杂志》2011年第7期737-740,共4页Chinese Journal of Neurosurgery

基　　金：国家自然科学基金面上项目（30970987）

摘　　要：目的 探讨电刺激嗅球对脑室下区（SVZ）神经干细胞（NSC）增殖、迁移及其分化的影响.方法 取SD大鼠48只,随机分为正常对照组、假刺激组、电刺激1 d、3 d、7 d、14 d、21 d、28 d组,以5-溴脱氧尿嘧啶（BrdU）标记NSC,免疫组化法检测SVZ区NSC的增殖情况.另取大鼠18只,随机分为正常对照组、假刺激组和电刺激组,以免疫组化法观察NSC向嗅球迁移情况,荧光双标法检测NSC分化情况.结果 电刺激1 d,SVZ区BrdU阳性细胞开始增高,第7天达高峰,第3周达对照组水平.注射BrdU28 d后,电刺激组喙侧迁移流（RMS）及嗅球内的BrdU阳性细胞数较正常对照组明显增多,但两组NSC分化情况没有明显差别.结论 电刺激嗅球可促进SVZ区NSC增殖及迁移,但对其分化没有影响.Objective To investigate the effects of electrical stimulation of olfactory bulb（ OB） on the proliferation, migration and differentiation of neural stem cell （ NSC ） in subventricular zone. Method Forty - eight adult female Sprague - Dawley（SD） rats were randomly divided into control group, sham stimulation group and stimulation group including 6 time points（1 day,3 day,7 day, 14 day,21 day, 28 day）. The rats were injected intraperitoneally with bromodeoxyuridine（ BrdU） to detect the proliferation of NSC by immunohistochemistry staining. Another 18 rats were randomly divided into control group, sham stimulation group and stimulation group. Four weeks after BrdU injection, the rats were sacrificed and immunohistochemistry and immunofluorescence were used to investigate the migration and differentiation of NSC in OB. Results In SVZ, BrdU - positive cells began to increase 1 d after stimulation （17. 67 ± 1.03, P 〈0.01） .reached to the maximum level at 1 week（28. 50 ± 1. 87, P 〈0. 01） ,then decreased to normal at 3 week. Four weeks after injection of BrdU,the BrdU -positive cells significantly increased in RMS（67. 33 ±3.50, P 〈0.01） and OB（44.33 ±5.47, P 〈0.01） in stimulation group. Fluorescence double staining showed that the stimulation of OB had no effect on the differentiation of NSC into neurons or gliocytes. Conclusions Electrical stimulation of OB could promote proliferation, migration of NSC in SVZ, but it has no effect on the differentiation of NSC into neurons or gliocytes.

关 键 词：电刺激 嗅球 神经干细胞 大鼠 

分 类 号：R363[医药卫生—病理学]