微小核糖核酸-25在胃癌患者血清中的异常表达及其临床意义  

The abnormal expression of microRNA-25 in the serum of gastric cancer patients and its clinicalsignification

作　　者：胡凤蓉[1] 帖君[1] 胡思隽[1] 刘清[1] 石晓磊[1] 聂勇战[1] 张宏博[1] 

机构地区：[1]第四军医大学西京医院消化内科国家肿瘤生物学重点实验室,西安710032

出　　处：《中华消化杂志》2011年第6期392-395,共4页Chinese Journal of Digestion

摘　　要：目的探讨微小核糖核酸-25（miRNA-25）在胃癌患者血清中的异常表达及其临床意义。方法选择第四军医大学西京医院手术及随访资料完整的胃癌（86例）、胃腺瘤（70例）患者和健康对照者（80名）为研究对象，提取血清总RNA，在建立了稳定、敏感的血清miRNA-25绝对定量检测方法（qRT-PCR）的基础上，检测胃癌、胃腺瘤患者和健康对照者血清中miRNA-25表达水平。分析胃癌、胃腺瘤和健康对照者血清中的miRNA-25表达差异及血清miRNA-25表达水平与胃癌临床病理参数的关系。结果胃癌患者血清中miRNA-25的表达水平（135．6ftool／μg总RNA）显著高于胃腺瘤（67．7fmol／μg总RNA）和健康对照者（62．2fmol／μg总RNA，P〈0．01），同时miRNA-25的受试者工作特征曲线显示，血清miRNA-25对胃癌诊断具有良好的特异度和敏感度（曲线下面积=0．827）；有淋巴结转移者[（148．3士10．2）fmol／μg总RNA]和临床病理分期Ⅲ／Ⅳ期[（146．7±9．5）fmol／μg总RNA]胃癌患者血清miRNA-25的表达分别明显高于无淋巴结转移者[（120．3士10．1）fmol／μg总RNA]和I／Ⅱ期胃癌患者[（119．4±12．2）fmol／μg总RNA]（P〈0．05）；相关性研究结果表明，血清miRNA-25高表达胃癌患者的生存期与血清miRNA-25低表达胃癌患者无明显差异（P〉0．05）。结论血清miRNA-25的检测可能有助于胃癌的诊断和预后预测。Objective To investigate the abnormal expression of microRNA （miRNA）-25 in the serum of gastric cancer patients and its clinical significance. Methods In Xijing Hospital of Digestive Diseases, Fourth Military Medical University, 86 gastric cancer patients with operation and completed follow-up data, 70 gastric adenoma patients and 80 healthy controls were selected as study objects. Total RNA was isolated from the serum. After the stable and sensitive miRNA-25 absolute quantity detection method established, the serum levels of miRNA-25 in gastric carcinoma patients, gastric adenoma patients and healthy controls were tested according to this method. The expression differences of miRNA-25 in the serum of patients with gastric cancer and gastric adenoma and healthy controls were analyzed with statistic analysis, and the correlation between miRNA-25 expression level and clinic pathological features of gastric cancer was also analyzed. Results The expression level of miRNA-25 in the serum of gastric cancer patients （135.6 fmol/μg total RNA） was significantly higher than that of gastric adenoma patients （67.7 fmol/μg total RNA） and healthy controls （62.2 fmol/μg total RNA）（P〈0.01）. The receiver operating characterisstic curve of miRNA-25 indicated that serummiRNA-25 with good specificity and sensitivity in gastric cancer diagnosis （AUC=0. 827）. The serum level of miRNA-25 in gastric cancer patients with lymph node metastasis [（148.3 ±10. 2） fmol/μg total RNA] or clinicopathological stage Ⅲ/IV patients [（146. 7 ±9.5） fmol/μg total RNA] was significantly higher than that of gastric cancer patients without lymph node metastasis [（120. 3±10. 1） fmol/μg total RNA] or clinicopathological stage I/Ⅱ patients [ （ 119.4±12. 2） fmol/μg total RNA] （ P〈0.05）. The correlation statistical analysis result indicated that there was no significant difference in survival period between serum miRNA-25 highly expressed and lowly expressed gastric cancer patients （P〉0.05）. C

关 键 词：微小RNAS 肿瘤标记 生物学 胃肿瘤 敏感性与特异性 预后 

分 类 号：R735.2[医药卫生—肿瘤]