狂犬病病毒3aG株核蛋白基因cDNA的克隆及序列分析  

Cloning and Sequencing of the Nucleoprotein Structural Gene cDNA of Rabies Virus Chinese 3aG Strain

作　　者：李伟[1] 张新梅[2] 张曼夫[1] 王树惠[2] 

机构地区：[1]中国农业大学生物学院,北京100094 [2]中国医学科学院基础医学研究所,北京100005

出　　处：《农业生物技术学报》1999年第3期223-229,共7页Journal of Agricultural Biotechnology

基　　金：九五"863"资助项目

摘　　要：从狂犬病病毒感染的ＢＨＫ 细胞裂解上清液中快速提取病毒ＲＮＡ，用反转录聚合酶链式反应（ＲＴＰＣＲ） 方法得到编码核蛋白完整结构基因的ｃＤＮＡ。进一步将此基因克隆入ｐＵＣ１８ 中，进行核苷酸序列分析，并与国外ＰＶ、ＣＶＳ、ＳＡＤＢ１９ 株以及国内５ａＧ 株的Ｎ 基因序列进行了同源性比较，证明所克隆Ｎ基因正确。The nucleoprotein structural gene cDNA of rabies virus 3aG strain was isolated by RT—PCR from genomic RNA. The PCR product was ligated into pUC18 plasmid vector and its nucleotide sequence was analyzed. The nucleotide sequence homology between 3aG strain and PV, CVS, SADB19 and 5aG strain was determined to be 92 46 %, 92 17 %, 92 69 %, 94 23 % respectively The deduced amino acid sequences between 3aG strain and PV, CVS, SADB19 and 5aG strain were 95 34 %, 94 46 %, 94 68 %, 96 01 % respectively No alteration of the phospharylation site at 389aa from N terminal was found No change in the sequential antigenic sites at 360~383aa from N terminal was discovered.

关 键 词：狂犬病病毒 核蛋白 CDNA 克隆 序列分析 

分 类 号：R373.9[医药卫生—病原生物学]