肿瘤坏死因子α对慢性粒细胞白血病源树突状细胞表达树突状细胞趋化因子1的影响  

Tumor necrosis factor-α influences level of dendritic cell-chemokine 1 expressed by chronic myeloid leukemia-derived dendritic cells

作　　者：李亮亮[1] 柴晔[1] 张连生[1] 曾鹏云[1] 吴重阳[1] 易良才[1] 陈红鹰[1] 白俊[1] 

机构地区：[1]兰州大学第二医院血液科,甘肃兰州730030

出　　处：《临床荟萃》2011年第15期1299-1302,F0002,共5页Clinical Focus

摘　　要：目的观察肿瘤坏死因子α(TNF-α)对体外诱导的慢性粒细胞白血病(CML)来源的树突状细胞(DCs)表达树突状细胞趋化因子1(DC-CK1)水平的影响。方法分离初诊20例CML患者外周血单个核细胞,用重组人粒-巨噬细胞集落刺激因子(rhGM-CSF)100μg/L、重组人白细胞介素4(rhIL-4)100μg/L培养5天,然后分为实验组和对照组两组,实验组加入TNF-α50μg/L,对照组只加培养基,第7天收获细胞。通过细胞形态学(倒置显微镜、Wright染色)及免疫表型对培养的DCs进行鉴定;荧光原位杂交(FISH)对DCs进行细胞遗传学检测;酶联免疫吸附测定(ELISA)法检测DC-CK1的表达水平。结果培养后实验组细胞的树枝样突起更明显,而且实验组DCs表面分子的表达比对照组上调,差异有统计学意义(P<0.05),同时经FISH证实了DCs的白血病来源;最后ELISA检测显示实验组DCs培养上清中DC-CK1的含量为(175.094±52.487)ng/L(n=10),明显低于对照组的(658.926±84.105)ng/L(n=10),差异有统计学意义(P<0.05)。结论 TNF-α抑制CML源树突状细胞表达DC-CK1。Objective To observe the influence of tumor necrosis factor-α（TNF-α） on the level of dendritic cell-chemokine 1（DC-CK1） expressed by dendritic cells（DCs） in vitro induced from chronic myeloid leukemia（CML）.Methods Peripheral blood monouclear cells（PBMC） were isolated from 20 newly diagnosed CML patients,and co-cultured with recombinant human granulocyte macrophage colony stimulating factor（rhGM-CSF） 100 μg/L and recombinant human interleukin-4（rhIL-4） 100 μg/L for 5 days,then TNF-α 50 μg/L was added to experimental group,control group only contained medium,the cells were harvested at the day 7th.DCs were studied by cell morphology（Wright staining,inverted microscope） and immunophenotype;fluorescence in situ hybridization（FISH） was used to analyze the cytogenetics.The level of DC-CK1 was measured by enzyme-linked immunosorbent assay（ELISA）.Results Dendrite-like projection of experimental group was more obvious than that of control group,and the expression of surface marker in experimental group was more up-regulated than that of control group,the difference had statistical significance（P0.05）.Meanwhile,the origin of leukemia was confirmed by FISH.ELISA showed that the level of DC-CK1 expressed by experimental group was（175.094±52.487） ng/L（n=10）,which was lower significantly than that of control group（658.926±84.105） ng/L（n=10）,and the difference had statistical significance（P0.05）.Conclusion TNF-α inhibited the generation of DC-CK1 expressed by CML-DCs.

关 键 词：白血病 髓样 慢性 肿瘤坏死因子Α 树突状细胞 趋化因子类 酶联免疫吸附测定 

分 类 号：R733.722[医药卫生—肿瘤]