HPLC-MS/MS法测定中药材枳壳中展青霉素  

Determination of patulin in zhike of Chinese herb by HPLC-MS/MS

作　　者：王少敏[1] 郑荣[1] 俞灵[1] 陈静[1] 季申[1] 王柯[1] 

机构地区：[1]上海市食品药品检验所,上海201203

出　　处：《中国卫生检验杂志》2011年第7期1593-1594,共2页Chinese Journal of Health Laboratory Technology

基　　金：国家科技重大专项课题项目(2009ZX09502-024)

摘　　要：目的:建立中药材枳壳中展青霉素的HPLC-MS/MS测定方法。方法:样品经果胶酶酶解,用水提取、经过展青霉素免疫亲和柱净化后,用高效液相色谱-三重四级杆串联质谱进行分析测定。结果:展青霉素在5.08 ng/ml～254.0 ng/ml范围内与峰面积呈良好的线性关系,线形方程为Y=2.17×103X-222,r=0.9977,回收率在80.0%～101.1%之间。在枳壳中最低检测限为0.02μg/kg。结论:本法灵敏、快速、准确、专属性强,可用于中药枳壳中展青霉素的测定。Objective：To establish a HPLC-MS/MS method for determination of Patulin in zhike of Chinese herb.Methods：After being enzyme-hydrolyzed by pectinase,extracted by water,and purified by patulin immune affinity column,Patulin were analyzed by HPLC-triple quadrupole MS.Results：There is a good linear relationship within the range of 5.08 ng·ml^-1～254.0 ng·ml^-1 for Patulin,with the linear equation Y=2.17×10^3X-222,r=0.9977.The recovery was between 80.0%～101.1%.The LOD in Zhike was 0.02 μg ·kg^-1 in minimum.Conclusion：The method is sensitive,rapid,accurate and specific enough to determine Patulin in Zhike.

关 键 词：展青霉素 高效液相色谱-串联三重四极杆质谱 枳壳 中药材 

分 类 号：O657.63[理学—分析化学]