鼻咽癌中转化生长因子β受体及受体相关蛋白1的原位表达  

The expression in situ of transforming growth factor βs,their receptors and TGFβ receptor interacting protein 1 in nasopharygneal carcinoma

作　　者：宾亮华[1] 胡成平[1] 湛凤凰[1] 曾朝阳[1] 曹莉 李桂源[1] 

机构地区：[1]长沙湖南医科大学肿瘤研究所,410078

出　　处：《中华耳鼻咽喉科杂志》1999年第4期210-212,共3页Chinese Journal of Otorhinolaryngology

基　　金：国家863 高技术计划资助

摘　　要：目的　研究转化生长因子β（ｔｒａｎｓｆｏｒｍｉｎｇ ｇｒｏｗｔｈｆａｃｔｏｒβ，ＴＧＦβ） 、其受体及受体相关蛋白１（ＴＧＦβｒｅｃｅｐｔｏｒｉｎｔｅｒａｃｔｉｎｇ ｐｒｏｔｅｉｎ１，ＴＲＩＰ１） 在鼻咽低分化鳞癌中的表达状况。方法　采用免疫组化技术及原位分子杂交技术，检测同一例标本中癌旁上皮和癌细胞内的３ 型ＴＧＦβ，２ 种受体的蛋白水平和ＴＲＩＰ１ ｍＲＮＡ水平，分析其变化规律。结果　ＴＧＦβ１、ＴＧＦβ２、ＴＧＦβ３、ＴＧＦβＲＩ、ＴＧＦβＲＩＩ在癌旁上皮表达强于癌组织的百分数分别为６５．７９％ 、６６．６７ ％ 、５５．２６％ 、４８．５７％ 、６３．１６ ％ 。癌旁上皮阳性率都高于癌组织阳性率（ Ｐ＜０．０１） ，癌细胞ＴＲＩＰ１ｍＲＮＡ表达平均灰度值１９．３２±１０．７０ ，癌旁上皮平均灰度值为１１．９６ ±５．８５（ Ｐ＜ ０．０５）。结论　鼻咽低分化鳞癌中ＴＧＦβ信息传递通路中的配体、受体和受体相关蛋白１ 表达减弱。Objective To study the expression of transforming growth factor(TGF)βs, their receptors and TGFβ receptor interacting protein 1(TRIP 1) in nasopharygneal carcinoma Methods Immunohistochemical technology and in situ hybridization methods were adopted to detect the TRIP 1 mRNA and 3 kinds of TGF β isoforms and 2 kinds of TGFβ receptors protein in the same biopsy specimen Results The positive expression of TGF β1, TGF β2, TGF β3, TGF βRⅠ and TGFβRⅡ was stronger in the tumor adjacent epithelium than in the tumor itself , which were 65 79%, 66 67%, 55 26%,4 8 57% and 63 16% higher than those in the tumor itself respectively( P <0 01) The level of TRIP 1mRNA measured in the epithelial cells was also higher than that in the tumor cells (19 32±10 70 versus 11 96±5 85, P <0 05) Conclusion The signal transmission of TGF β family is diminished in the poorly differentiated squamous cell carcinoma of nasopharynx It may be a factor for the development of nasopharygneal carcinoma

关 键 词：鼻咽肿瘤 转化生长因子Β 原位杂交 免疫组织 

分 类 号：R739.630.3[医药卫生—肿瘤]