威灵仙多糖对人乳腺癌MCF-7细胞凋亡及Bcl-2,Fas基因表达的影响  

The effect of radix clematidis polysaccharide on the apoptosis and Fas,Bcl-2 gene Expresion of MCF-7 human breast cancer cells

作　　者：张杰[1] 杨旭东[1] 赵容杰[1] 

机构地区：[1]黑龙江省牡丹江医学院,黑龙江牡丹江157011

出　　处：《中国优生与遗传杂志》2011年第8期17-18,22,共3页Chinese Journal of Birth Health & Heredity

基　　金：黑龙江省卫生厅科学课题(2009-413)

摘　　要：目的探讨威灵仙多糖对人乳腺癌MCF-7细胞凋亡的影响及其分子机制。方法不同浓度威灵仙多糖作用于MCF-7细胞48h后,荧光显微镜观察人乳腺癌细胞MCF-7细胞凋亡情况,RT-PCR法检测Bcl-2、Fas基因表达量的变化。结果威灵仙多糖能诱导MCF-7细胞凋亡,40mg/L时凋亡率为17.63%,实验组与对照组比较有显著性差异;并且上调Fas和降低Bcl-2表达。结论威灵仙多糖能抑制MCF-7细胞增殖,促进其细胞凋亡,其机制可能与激活Fas、抑制Bcl-2的基因表达有关。Objective：To investigate the influence of Radix Clematidis polysaccharide（RCP）on apoptosis of MCF-7 cells and reveal the mechanism.Methods：MCF-7 cells were treated with various of RCP,we observed cell apoptosis of MCF-7 cells by fluorescence microscope.We detected the levels of Bcl-2 and Fas gene expression in all groups by RT-PCR.Results：RCP has obviously apoptosis-inducing effects on MCF-7 cells.And apoptotic rate was 17.63%when the density of RCP was 40mg/L.It was found that there was a significant difference between the experiment groups and control group.It could effectively decrease the level of Bcl-2 gene expression and recrease the level of Fas gene expression.Conclusion：RCP could inhibit the growth and induce apoptosis of MCF-7 cells probably through down regulating Bcl-2 gene expression and up regulating Fas gene expression.

关 键 词：威灵仙多糖 乳腺癌 FAS BCL-2 

分 类 号：R737.9[医药卫生—肿瘤]