内皮素-1(ET-1)对羊驼皮肤黑素细胞增殖和黑素生成的影响  

Effect of ET-1 on Proliferation and Melanin Synthesis of Alpaca Skin Melanocytes in vitro

作　　者：耿建军[1] 张杰[1] 穆晓丽[1] 白瑞[1] 董彦君[1] 白俊明[1] 董常生[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801

出　　处：《畜牧兽医学报》2011年第7期932-938,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30571070);山西省自然科学基金(2007011082)

摘　　要：旨在研究内皮素-1(Endothelin-1,ET-1)对羊驼皮肤黑素细胞(Melanocyte,MC)增殖和黑素合成的影响。本研究中,体外培养正常羊驼皮肤黑素细胞,观察不同浓度ET-1(0、0.1、1、10、100nmol.L-1)对羊驼皮肤黑素细胞增殖、黑素含量、内皮素受体B(Endothelin recepter B,EDNRB)基因、酪氨酸酶(Tyrosinase,TYR)基因、酪氨酸相关蛋白-1(Tyrosinase related protein 1,TRP-1)基因和表皮黑皮素-1受体(Melanocortin 1receptor,MC1R)基因表达量的影响。结果表明,ET-1处理羊驼皮肤黑素细胞3d后,羊驼皮肤黑素细胞增多,黑素含量、EDNRB、TRP-1和TYR基因表达量都明显增加(P<0.05),以10nmol.L-1组最为显著。ET-1能诱导羊驼皮肤黑素细胞增殖、树突增长,诱导EDNRB、TYR和TRP-1基因表达量增高,使黑素合成增加;同时诱导MC1R基因表达量增高,从而通过α-MSH信号通路对羊驼黑色素的生成产生影响。To study the effects of Endothelin-1（ET-1） on the proliferation and melanin synthesis of cultured alpaca skin melanocytes,the cultured alpaca skin melanocytes were treated with various concentrations of ET-1（0,0.1,1,10,100 nmol·L-1） in vitro.The effects of ET-1 on melanocyte proliferation,melanin contents,EDNRB,TYR,TRP-1 and MC1R gene expeession in cultured melanocytes were observed.Three days after treatment with ET-1,the number of melanocytes increased and melanin contents,EDNRB,TYR and TRP-1 gene expression levels also remarkably increased in treated cells compared to those in untreated cells（P〈0.05）,especially when the concentration was 10 nmol·L-1.The result indicate that ET-1 can induce the dendrites elongated,proliferation and the expression of EDNRB,TYR and TRP-1,and induce the melanin synthesis;Also,ET-1 induce the expression of MC1R gene,and affect the melanin synthesis of alpaca by the pathway of α-MSH.

关 键 词：ET-1 羊驼 皮肤 黑素细胞 EDNRB TYR MC1R TRP-1 

分 类 号：S813.3[农业科学—畜牧学]