大鼠骨髓间充质干细胞分化的神经细胞在体外对C6胶质瘤细胞的趋向性  

The rat bone marrow mesenchymal stem cells differentiate into neural cells and it's tropism towards C6 glioma cells in vitro

作　　者：钟俊[1] 刘卫平[1] 龙乾发[1] 刘阳[2] 王孝安[1] 韩蕊[3] 

机构地区：[1]第四军医大学西京医院神经外科,陕西西安710032 [2]绵阳市第三人民医院神经外科,四川绵阳621000 [3]武警陕西总队医院眼科,陕西西安710054

出　　处：《国际神经病学神经外科学杂志》2011年第3期212-216,共5页Journal of International Neurology and Neurosurgery

基　　金：四川省卫生厅科学研究项目:创伤性脑损伤后bFGF干预骨髓间充质干细胞治疗作用的对比试验研究2010-100301;陕西省科技厅科学技术研究发展计划项目:神经干细胞基因转染对脑胶质瘤的治疗2008K09-09

摘　　要：目的研究大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)诱导分化后的神经细胞在体外对C6胶质瘤细胞的趋向性。方法首先采用梯度离心分离BMSCs,在条件培养基中添加合适诱导因子诱导BMSCs分化为神经细胞,分化后的细胞进行免疫荧光细胞化学分析后与C6胶质瘤细胞采用Transwell培养板共培养,最后对迁移的细胞做统计学分析。结果 BMSC成功诱导分化为神经细胞Nestin(24.3±5.2)%、NSE(33.6±3.8)%和NeuN(41.9±4.7)%,共培养实验显示实验组迁移细胞明显多于对照组迁移细胞(p<0.05)。结论大鼠骨髓间充质干细胞分化后的神经细胞在体外对C6胶质瘤细胞具有明显趋向性。Objective To study the tropism of rat bone marrow mesenchymal stem cells（BMSCs） differentiated into neural cells towards C6 glioma cells in vitro.Methods Firstly BMSCs were induced to differentiated into neural cells through adding the appropriate inducing factors into condition medium（such as 2% B27,20ng/ml bFGF,20ng/mlEGF and so on）,and then the neurally differentiated BMSCs and C6 glioma cells were co-cultured with transwell system.The number of migrated cells was counted by statistical software.Results Rat BMSCs successfully differentiated into neural cells（Nestin（24.3±5.2） %,NSE（33.6±3.8） % and NeuN（41.9±4.7） %）,and transwell co-culture test manifested the number of migrated cells in experimental group was more significant than the which in control group.Conclusions Neurally differentiated rat BMSCs have obviously tropism towards C6 glioma cells in vitro.

关 键 词：骨髓间充质干细胞 神经细胞 C6胶质瘤细胞 

分 类 号：R739.4[医药卫生—肿瘤]