中脑黑质多巴胺神经元对适当剂量鱼藤酮毒性损伤的特殊敏感性  

The special sensitivity of substantia nigra dopaminergic neurons to toxic injury of appropriate dose of rotenone

作　　者：张玉梅[1] 于德钦[2] 彭岩[2] 吴雪飞[2] 张万琴[2] 赵杰[2] 

机构地区：[1]辽宁中医药大学附属医院中医内科,沈阳110032 [2]大连医科大学生理学教研室,大连116044

出　　处：《神经解剖学杂志》2011年第4期377-382,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(30770737)

摘　　要：目的:探索中脑黑质多巴胺神经元对适当剂量鱼藤酮毒性损伤是否具有特殊敏感性。方法:采用颈背部皮下注射鱼藤酮的方法建立大鼠中脑黑质多巴胺神经元损伤模型,进行中脑黑质和纹状体酪氨酸羟化酶(TH)免疫组织化学染色加尼氏染色;同时通过HE染色及尼氏染色方法分别观察心、肝、脾、肾等重要胸腹腔脏器及海马、顶叶皮质的形态学变化。结果:中脑黑质TH免疫染色和尼氏染色结果显示组间中脑黑质致密区以外部位尼氏小体数目无差异;大脑顶叶皮质和海马尼氏染色及胸腹腔重要脏器HE染色结果表明各组大鼠均未出现相应部位损伤。结论:低剂量颈部皮下注射鱼藤酮能选择性诱导中脑黑质多巴胺神经元损伤,说明中脑黑质多巴胺神经元对鱼藤酮具有高度敏感性。Objective： To explore the special sensitivity of dopaminergic neurons in substantia nigra to toxic injury with the appropriate dose of rotenone.Methods： After establishing dopaminergic neurons in substantia nigra injury model by injecting rotenone subcutaneously on back of neck,immunohistochemistry for tyrosine hydroxylase（TH） in substantia nigra and striatum and Nissl staining were carried out.Also,hematoxylin-eosin（HE） staining and Nissl staining were used to observe the morphological changes in heart,liver,spleen,kidney,hippocampus and parietal cortex.Results： Immunohistochemistry for TH and Nissl staining showed that no difference in Nissl bodies were observed in brain areas outside the substantia nigra part in different groups.HE staining and Nissl staining results also showed that no difference could be seen in heart,liver,spleen,kidney,hippocampus and parietal cortex.Conclusion： Subcutaneous injection with low-dose rotenone could selectively induce dopamine neurons damages in substantia nigra.The data suggest that dopaminergic neurons is highly sensitive to rotenone.

关 键 词：多巴胺神经元 帕金森病 鱼藤酮 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]