精神分裂症模型小鼠脑内c-Fos/NADPH-d的表达  

Expressions of c-Fos and NADPH-d in the brain of schizophrenia model mice

作　　者：马传响[1] 宋亮[2] 刘阳[2] 刘芳[1] 

机构地区：[1]徐州医学院人体解剖学教研室,徐州221004 [2]徐州医学院神经生物学教研室,徐州221004

出　　处：《神经解剖学杂志》2011年第4期423-427,共5页Chinese Journal of Neuroanatomy

基　　金：徐州市科技计划项目(XM07C057)

摘　　要：目的:检测精神分裂症模型小鼠脑内c-Fos与尼克酰胺腺啶呤二核苷酸磷酸黄递酶(NADPH-diapho-rase,NADPH-d)的共存,探讨一氧化氮(nitricoxide,NO)与精神分裂症的关系,为深入研究精神分裂症的发病机制提供形态学和神经化学依据。方法:雄性昆明小鼠,随机分为生理盐水组和苯环己哌啶(phencyclidine,PCP)组,分别用自主活动观察、Sams-Dodd的刻板行为评分标准对模型小鼠的行为改变进行检测,同时用NADPH-d组织化学和c-Fos免疫组织化学双染法检测各组小鼠脑内c-Fos/NADPH-d阳性神经元的分布。结果:(1)PCP组小鼠出现明显的自发活动的增加(P<0.01)及显著的刻板行为(P<0.01);(2)PCP组小鼠脑内c-Fos与NADPH-d阳性神经元单纯表达的数量明显高于对照组,并且在伏隔核、屏状核及被盖背外侧核出现较多c-Fos/NADPH-d双标阳性神经元,生理盐水组小鼠脑内未见c-Fos/NADPH-d双标阳性神经元。结论:NO可能在谷氨酸NMDA受体功能异常引起的精神分裂症的病理过程发挥一定作用。Objective： To investigate the relationship between nitricoxide NO and the schizophrenia by detecting the expressions of c-Fos/ NADPH-d in the mouse brain,and to provide the evidence of morphology and neurochemistry for nosopoietic mechanism of schizophrenia.Methods： Male Kunming mice were randomized into two groups： normal saline group and phencyclidine（PCP） group.Their ethological changes were determined by spontaneous activity observation and Sams-Dodd stereotyped behavior scores respectively.Meanwhile,labeling with histochemistry-immunohistochemistry was performed to observe c-Fos/NADPH-d positive neurons of each group.Results：（1）Compared with the normal saline group,the PCP group mice had more spontaneous activities（P〈0.01）,and showed more significant stereotype behavior（P〈0.01）;（2）The number of simple c-Fos or NADPH-d single labeled neurons in the PCP group was significantly higher than that in normal saline group.More c-Fos/NADPH-d double-labeled neurons in the Acb（accumbens nucleus）,Cl（claustrum） and LDTg（laterodorsal tegmental nucleus） were observed in the PCP group,while negative in the normal saline group.Conclusion： NO may play a role in the pathological process of schizophrenia caused by abnormal glutamate NMDA receptor function.

关 键 词：精神分裂症 小鼠 C-FOS NADPH-D NO 

分 类 号：R749.3[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]