大鼠短暂性局灶性脑缺血早期HMGB1在半暗带区的表达  

Early accumulation of high-mobility group box 1 protein in penumbra region of the brain following transient middle cerebral arterial occlusion in the rat

作　　者：王鄂友[1] 朱正华[2] 朱萧玲[2] 夏中元[1] 熊利泽[2] 

机构地区：[1]武汉大学人民医院麻醉科,武汉430060 [2]第四军医大学西京医院麻醉科,西安710032

出　　处：《神经解剖学杂志》2011年第4期438-442,共5页Chinese Journal of Neuroanatomy

摘　　要：目的:探讨调节炎症反应的细胞因子HMGB1在脑缺血/再灌注损伤中的时程变化特点。方法:采用大鼠MCAO模型,利用ELISA及免疫组织化学染色方法观察缺血/再灌注后不同时间点脑内HMGB1蛋白的表达变化。结果:ELISA检测提示再灌注后大鼠缺血侧大脑HMGB1表达显著增高,在10 h和70 h形成两次高峰(P<0.05);免疫组织化学染色提示再灌注1 h后部分大鼠(1/5)和再灌注后4,10,22和70 h后全部大鼠梗死区周边半暗带区的细胞外间隙有HMGB1阳性染色颗粒,后期并在部分细胞内出现HMGB1高表达。结论:结果提示,HMGB1不仅与脑缺血/再灌注的晚期损伤有关,亦可能参与了早期损伤过程。Objective： To explore the time-course of the expression of High-mobility group box 1（HMGB1） protein which have recently been identified as a late cytokine mediator of sepsis and inflammation in the brain following ischemia-reperfusion injury.Methods： The appearance and location of HMGB1 protein in rat brain following transient cerebral ischemia by the middle cerebral artery occlusion with silicon-coated thread for 2 h was investigated by ELISA and immunohistochemical staining.Results： The expression of HMGB1 significantly increased in ischemic hemisphere compared to the non-ischemic hemisphere,and developed two peak at 10 h and 70 h respectively after MCAO.HMGB1 positive staining particles were observed in extracellular milieu in the penumbra zone surrounding the infarct area in one of the five rats at 1 h after reperfusion,and in all of the rats at 4,10,22 and 70 h（n=5,each time point） after reperfusion.Cells containing HMGB1 positive particles in their cytoplasm appeared in necrotic regions only at 70 h after reperfusion.No HMGB1 positive particles were observed in the non-ischemic brain hemispheres.Conclusion： The findings indicate that HMGB1 is not only involved in the late phase but also the early phase injury after brain ischemia-reperfusion.

关 键 词：高迁移率族蛋白B1 脑缺血 细胞因子 炎症 半暗带 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]