姜黄素联合奥沙利铂对人胃癌细胞生长及其X连锁凋亡抑制蛋白表达的影响  

influence of curcumin combined with oxaliplatin on the growth and expression of X-linked inhibitor of apoptosis protein of human gastric cancer cells

作　　者：曾呈军[1] 胡金喜[1] 卢明东[1] 李丕宏[1] 刘磊[1] 林赛锋[1] 俞耀军[1] 王飞海[1] 郑志强[1] 

机构地区：[1]温州医学院附属第二医院普外科,浙江温州325000

出　　处：《温州医学院学报》2011年第4期315-320,共6页Journal of Wenzhou Medical College

基　　金：浙江省中医药科研基金资助项目(2006C180)

摘　　要：目的:观察姜黄素与奥沙利铂对人胃癌细胞株SGC-7901生长的影响,并探讨其与X连锁凋亡抑制蛋白(XIAP)的关系。方法:细胞生长抑制试验(MTT)法测定不同浓度的姜黄素与奥沙利铂对SGC-7901细胞增殖的影响;流式细胞术检测细胞凋亡率;Hoechst33258染色观察细胞凋亡形态学改变;荧光定量RT-PCR检测XIAP mRNA的表达;分光光度法检测Caspase-3的活性。结果:姜黄素和奥沙利铂均能抑制SGC-7901细胞的增殖,呈现浓度依赖性,联合作用组的抑制率显著高于姜黄素组和奥沙利铂组(均P<0.01);两药均能诱导胃癌SGC-7901凋亡,联合作用组的细胞凋亡率显著高于姜黄素组和奥沙利铂组(均P<0.01);两药均能抑制XIAP mRNA的表达,联合作用组中XIAP mRNA的表达显著低于姜黄素组和奥沙利铂组(均P<0.01);两药均能提高Caspase-3的活性,联合作用组中Caspase-3的活性显著高于姜黄素组和奥沙利铂组(均P<0.01)。结论:姜黄素和奥沙利铂均能抑制SGC-7901细胞的增殖、诱导凋亡,两者联合作用效果更加显著,这可能与两者均能抑制XIAP表达、提高Caspase-3的活性有关。Objective： To observe the effect of curcumin and oxaliplatin on the growth of human gastric cancer cells SGC-7901 and its relationship to X-linked inhibitor of apoptosis protein（XIAP）.Methods： MTT assay was performed to detect the proliferation after the cells were treated with different concentrations of curcumin and oxaliplatin.Flow cytometry was performed to detect the apoptosis rate.Hoechst33258 staining was used to observe the morphological changes of apoptosis.The expression of XIAP mRNA was analyzed with qRT-PCR.The activation of Caspase-3 was detected with spectrophotometry.Results： Both of curcumin and oxaliplatin could inhibit the proliferation of SGC-7901 cells in a concentration-dependent manner and the inhibition rate of the combined groups was significantly higher than that of the curcumin or oxaliplatin treated groups（all P 0.01）.The two drugs could induce apoptosis of human gastric cancer cells SGC-7901 and the apoptosis rate of the combined group was significantly higher than that of the curcumin or oxaliplatin treated groups（both P 0.01）.The two drugs could inhibit the expression of XIAP mRNA,and the expression of XIAP mRNA in the combined group was significantly lower than that in the curcumin or oxaliplatin treated groups（both P 0.01）.The two drugs could increase the activation of Caspase-3,and the activation of Caspase-3 in the combined groups was significantly higher than that in the curcumin or oxaliplatin treated groups（both P 0.01）.Conclusion： Both of curcumin and oxaliplatin can inhibit the proliferation of gastric carcer cells SGC-7901 and induce apoptosis.The combination of them can strengthen these effects possibly by inhibiting the expression of XIAP mRNA and increasing the activation of Caspase-3.

关 键 词：姜黄素 奥沙利铂 胃肿瘤 X连锁凋亡抑制蛋白 

分 类 号：R735.2[医药卫生—肿瘤]