不同转移潜能人肺癌细胞系Ezrin表达水平及微丝骨架差异性的研究  

Ezrin expression and actin filament organization in lung cancer cell lines with different metastatic potential

作　　者：陈清勇[1] 严杰[1] 胡慧珍[1] 陈方园[2] 宋嘉[1] 吴玉泉[1] 焦德敏[1] 

机构地区：[1]解放军第117医院呼吸内科,浙江杭州310004 [2]浙江中医药大学09级研究生,浙江杭州310053

出　　处：《中华肿瘤防治杂志》2011年第13期1013-1016,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：浙江省医药卫生科学研究基金(2008A0144)

摘　　要：目的:探讨Ezrin表达水平与肺癌转移潜能的相关性以及细胞内微丝骨架的变化差异。方法:应用蛋白质印迹法和RT-PCR方法检测4种具有不同转移潜能的人类肺癌细胞系中Ezrin mRNA和蛋白的表达水平;荧光染色的方法显示细胞内微丝骨架的变化。结果:Ezrin的mRNA和蛋白表达水平在高转移潜能的人类肺癌细胞中高于相应的低转移肺癌细胞;划痕修复实验显示,高转移组细胞的划痕修复能力明显增强。95D和801D细胞的划痕修复率〔(52.15±5.21)%和(54.22±4.55)%〕明显高于95C和A549细胞的划痕修复率〔(20.15±4.85)%和(21.33±5.01)%〕,差异有统计学意义,P<0.01。高转移性肺癌与低转移肺癌细胞比较,前者细胞内肌动蛋白多聚体减少,微丝骨架杂乱、模糊,而低转移肺癌细胞和肺成纤维细胞微丝骨架粗大、结构清晰、排列整齐。结论:Ezrin mRNA和蛋白表达水平与肺癌的转移能力呈正相关性;肺癌转移潜能的增高伴有肌动蛋白多聚体的丢失和微丝结构的解聚,微丝骨架的这种变化与Ezrin的表达增高具有相关性。OBJECTIVE：To investigate the expression of Ezrin and related changes of actin filament organization in human lung cancer cell lines with different metastatic potential.METHODS： Four human lung cancer cell lines with different metastatic potential were analyzed for the expression of Ezrin detected by western blot and RT-PCR.The filamentous actin（F-actin） was stained with TRITC-phalloidin to detect changes in actin organization.RESULTS：In comparison with the lowly metastatic counterparts,Ezrin was up-regulated in highly metastatic human lung cancer cell lines.It was statistically significant of the scratches repair rate in both cell groups.It was more significant in the highly metastatic cells（（52.15±5.21）% in 95D cells,and（54.22±4.55）% in 801D cells,respectively） compared with lowly metastatic cells（（20.15±4.85）% in 95C cells and（21.33±5.01）% in A549 cells,respectively）.Further study indicated that over-expressing Ezrin in lung cancer cells had more motile.Furthermore,TRITC-phalloidin staining revealed less actin bundles and a fuzzy network of shorter filaments in the highly metastatic tumor cells,while in the non and/ or weakly metastatic cancer cell lines,there were thick and orderly arranged actin filaments.CONCLUSIONS： Ezrin levels correlate positively with the metastatic phenotype in human lung cancer cell lines.The increased metastatic potential of tumor cells is accompanied by the loss of F-actin and poorly organized actin skeleton.There is a consistent correlation between the elevated Ezrin expression and the disrupted actin skeleton.

关 键 词：EZRIN 肺肿瘤 转移 细胞运动 微丝 

分 类 号：R734.2[医药卫生—肿瘤]