神经生长因子对脂多糖诱导的成骨细胞炎症反应的影响  

Effect of nerve growth factor on lipopolysaccharide-induced osteoblast inflammation

作　　者：汪琼[1] 路晓淼[2] 张红园[3] 梅玲[1] 张菊会[3] 王恩群[3] 沈园[1] 李俊杰[1] 

机构地区：[1]安徽医科大学,230032 [2]蚌埠医学院第一附属医院口腔科 [3]安徽医科大学附属安庆医院口腔科

出　　处：《现代口腔医学杂志》2011年第4期290-293,共4页Journal of Modern Stomatology

基　　金：安徽省自然科学基金(070413096)

摘　　要：目的研究外源性神经生长因子(NGF)对脂多糖(LPS)诱导的成骨细胞炎症相关基因表达的影响。方法原代培养新生大鼠颅盖骨成骨细胞,Griess试剂法测定培养基上清中NO含量:MTT法检测细胞活力;Real-time定量PCR检测炎症相关基因TNF-α、COX-2和NF-κB mRNA表达。结果 NGF高中剂量(100ng/ml、10ng/ml)可显著抑制LPS诱导的成骨细胞NO的释放,该浓度下细胞活力不受影响;NGF可显著抑制TNF-α、COX-2和NF-κB基因表达。结论 NGF通过减少NO释放,抑制炎症相关基因表达发挥抗炎作用。Objective To evaluate the effect of exogenous nerve growth factor（NGF） on the relevant gene expression of lipopolysaccharide-induced osteoblast inflammation.Methods The neonatal rat calvarial osteoblast cells in primary culture were cultured;the NO content of supernatant in culture medium was determined with Griess method;the cell viability was tested with MTT method;and the inflammation related gene expressions of TNF-α,COX-2 and NF-κB mRNA was tested by means of Real-time analysis.Results The high-and middle-dose NGF may significantly inhibit the release of LPS-induced osteoblast NO,with no effect on cell viability in such concentration;NGF can remarkably inhibit the gene expression of TNF-α,COX-2 and NF-κB.Conclusion NGF exerts an anti-inflammatory action by inhibiting the relevant gene expression and reducing the release of NO.

关 键 词：神经生长因子 成骨细胞 脂多糖 TNF-α COX-2 NF-ΚB 

分 类 号：R780.2[医药卫生—口腔医学]