荧光谱客观分析在膀胱癌光动力学诊断中的应用  

Objective analysis of fluorescence spectrum using in photodynamics diagnosis of bladder cancer

作　　者：邹钧[1] 毕学成[2] 何慧婵[2] 姚永帮[3] 韩兆冬[2] 叶永康[2] 梁宇翔[2] 钟惟德[2] 

机构地区：[1]广州医学院第三附属医院泌尿外科,510150 [2]广州医学院附属广州市第一人民医院泌尿外科 [3]中国科学院安徽光学精密机械研究所

出　　处：《中华生物医学工程杂志》2011年第2期166-169,共4页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金（30872960）

摘　　要：目的 探讨紫外激光激发膀胱癌组织细胞荧光谱分析对改进膀胱癌光动力学诊断的意义.方法 以不含细胞培养液和血卟啉单甲醚混合液为对照,分别取浓度为0、10、100、200 mg/L的血卟啉单甲醚作为光敏剂,孵育膀胱癌组织细胞株T24 1～3 h,再以全固态紫外激光作为激发光源（波长355 nm）激发,检测膀胱癌组织细胞特征荧光光谱并分析药物峰位置.结果 在可见光区域390～780 nm,不含细胞的培养液和血卟啉单甲醚混合液未出现特征峰;无血卟啉单甲醚光敏剂的膀胱癌组织细胞的自体峰在445～490 nm处;不同时间不同浓度血卟啉单甲醚光敏剂孵育的膀胱癌组织细胞除在445～490 nm处有自体峰外,在628.65 nm处均出现药物峰,200 mg/L血卟啉单甲醚孵育2 h时膀胱癌组织细胞药物峰及自体峰荧光强度达到最高,且药物峰的荧光强度超过了自体峰.结论 紫外激光激发的膀胱癌组织细胞荧光谱性质稳定、强度佳,对改进膀胱癌光动力学诊断具有确切价值.Objective To explore the significance of fluorescence spectrum of bladder cancer tissue cells induced by ultraviolet light for improvement of photodynamics diagnosis in bladder cancer.Methods A cell-free mixture of culture medium and photosensitizer（Hematoporphyrin monomethyl ether,HMME）was used as the control.The cells line T24 of bladder cancer tissue were incubated with different concentrations of HMME（0,10 mg/L,100 mg/L and 200 mg/L）for 1 to 3 hours.Thereafter,the specific fluorescence spectrum and the drug peak position of bladder cancer were detected using a 355 nm full solidstate ultraviolet laser for excitation.Results The cell-free mixture of culture medium and HMME did not demonstrate any specific spectrum within the range of visible light（390-780 nm）.An inherent peak was shown in samples of HMME-free（0 mg/L）bladder cancer cells at 445-490 nm,whereas a drug peak was shown constantly at 628.65 nm in HMME-treated bladder cancer tissues,regardless of incubation time and doses of HMME.Highest level of inherent and drug peaks were noted in cell samples subjected to incubation with 200 mg/L HMME for 2 hours,where the drug peak Was even higher than the inherent one.Conclusion Fluorescence spectrum of bladder cancer tissue cells induced by ultraviolet laser demonstrates stable property and high intensity,which can be definitely promising for the improvement of photodynamics diagnosis in bladder cancer.

关 键 词：膀胱肿瘤 光谱法 荧光 紫外线 激光 固体 光动力学 

分 类 号：R737.14[医药卫生—肿瘤]