Orexin-A对戊四氮慢性点燃癫痫大鼠学习记忆功能的影响  

Effect of orexin-A on learning and memory of pentyleneterazoi-kindled epileptic rats

作　　者：杨位霞[1] 倪丽艳 唐吉友[3] 

机构地区：[1]复旦大学附属中山医院青浦分院神经内科,上海201700 [2]菏泽市巨野县人民医院神经内科 [3]山东大学附属千佛山医院神经内科

出　　处：《中华行为医学与脑科学杂志》2011年第7期633-635,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：山东省自然科学基金项目（Y2008C161）

摘　　要：目的探讨Orexin—A对戊四氮（PTZ）慢性点燃癫痫大鼠学习记忆功能的影响。方法健康成年雄性Wistar大鼠随机分为对照组（生理盐水）和戊四氮组。点燃成功后每组分别脑室内注射10μlorexin-A（1．4nmol／μl）或等量生理盐水，利用Morris水迷宫实验进行学习记忆能力检测。结果致痫组戊四氮点燃癫痫的成功率达80％。与非致痫组比较，慢性点燃大鼠搜寻平台的潜伏期明显延长（F=200．956，P〈0．01）；大鼠在平台所在象限的游泳时间和120s内穿越平台区域的次数显著减少（P〈0．01）。脑室内注射orexin-A后，航行定位实验中致痫组大鼠逃避潜伏期明显缩短（P〈0．01），非致痫组大鼠的逃避潜伏期也明显缩短（P〈0．05）。在空间搜寻实验中，orexin-A能明显增加穿越平台象限的次数[（10．83±1．84），（4．67±3．34）]和平台所在象限的游泳时间[（39．73±2．03）S，（33．76±2．96）S]，尤其是对致痫组大鼠的作用更明显（P〈0．01），搜寻站台的效率明显提高。结论Orexin—A能改善慢性点燃癫痫大鼠的空间学习记忆能力。Objective To investigate the effects of orexin-A on learning and memory of pentyleneterazol （PTZ）-kindled epileptic rats. Methods Adult Wistar rats were randomly divided into control group （normal saline, NS） and PTZ group. The PTZ-kindled rats were randomly divided into,orexin-A group and NS group administrated by intracerebroventricular （i. c. v. ） injection of 101xl orexin-A（ 1.4 nmol/μl） or 10μl NS. Using Morris water maze experiment,the ability of learning and memory was measured in all rats. Results Eighty percent of rats in PTZ group were kindled successfully after intraperitoneal injection of 35mg/kg PTZ every day for 30 days. Compared to control group, the escape latency in the PTZ-kindled rats was significantly increased in place navigation test （PNT） （F = 200.956, P 〈 0.01 ）, whereas a remarkable reduction of time spent in the target quadrant and number of pool circlings in 120 seconds was observed during probe trials. Following injection of orexin-A,the latency of escape platform was significantly declined in both PTZ-kindled （ （39.73 ± 2.03 ） s, （ 33.76 ± 2.96 ） s） and NS rats,increased the number of crossing the platform（ 10.83 ± 1.80） vs （4.67 ± 3.34）. In addition, the treatment with orexin-A markedly increased swim velocity and number of pool circlings in both groups （P 〈 0.01 ）, particularly to the PTZ-kindled rats. Conclusion Spatial learning and memory in the PTZ-kindled rats can be improved by treatment with orexin-A.

关 键 词：癫痫 OREXIN-A 学习和记忆 

分 类 号：R742.1[医药卫生—神经病学与精神病学]