局灶性脑缺血大鼠TGF-β_1免疫组化研究  

Increased Expression of TGF-β_1 in Experimental Rat Following Middle Cerebral Artery Occlusion

作　　者：杨四清[1] 黄怀钧[1] 王凤霞[1] 

机构地区：[1]湖北医科大学附属第二医院神经科,武汉430071

出　　处：《医学新知》1999年第4期175-178,共4页New Medicine

摘　　要：实验采用阻断一侧大脑中动脉（MCAO）造成局灶性脑缺血模型，运用免疫组织化学方法测定脑缺血1h及3h脑组织中的TGF－β1阳性染色状况。结果显示：①脑缺血1h后缺血测与缺血对侧、缺血侧与对照组的TGF－β1染色均有显著性差异（P＜0．05）；而缺血中心和缺血半影区的比较无计学意义（P＞0．05）；②脑缺血3h后的TGF－β1表达较缺血1h有新的变化，缺血半影区阳性率呈上升趋势而缺血中心则呈下降趋势（93．3％vs100％，73．3％vs60％），其变化具有显著性差异（P＜0．05）。表明脑缺血后神经细胞TGF－β1表达增高及TGF－β1可能参与神经细胞抗损伤机制。In this experiment, using the model of focal cerebral ischemia by occluding the middle cerebral artery in rat, We observed TGF - β1 staining in its brain which had been occluded for 1 hour or 3 hours by immunohistochemistry. The results showed: 1.the expression of TGF - β1 in the group one (occlueded for 1 hour) was significant difference between infarct versus conrtolateral hemisphere and infarct versus control subject (P <0. 05), otherwise there was no significance between ischemic core and ischemic penumbra(IP). 2. the expression of TGF - β1 in the group two (occluded for 3hours) was highly significant difference between infarct versus non - ischemic subject and infarct versus conrtol subJect (P <0. 01 ), and there was significant differerce between ischemic core and IP. Conclusion: expression of TGF - β1 in rat following PMCAO had increased and it might be of neuroprotective value.

关 键 词：脑缺血 TGF-Β1 免疫组织化学 缺血半影区 

分 类 号：R743.303[医药卫生—神经病学与精神病学]