高免卵黄透析液转移迟发型超敏反应的实验研究  

The Study of Hyperimmune Egg Yolk Dialysates on Transferring Delayed Type Hypersensitivity

作　　者：陈德元[1] 谢名荣[1] 徐刚[1] 黄树其 朱平 殷志新 

机构地区：[1]中国人民解放军广州医学高等专科学校,510315

出　　处：《中国生化药物杂志》1999年第6期281-282,共2页Chinese Journal of Biochemical Pharmaceutics

基　　金：广东省中间试验项目课题

摘　　要：建立一种新的制备特异性转移因子的方法。方法：用二硝基氯苯（ＤＮＣＢ）免疫鸡，当观察到迟发型超敏反应发生后，收集鸡蛋制备ＤＮＣＢ高免卵黄透析液，并将其注入小鼠腹腔内，６ ｄ后用 ＤＮＣＢ攻击小鼠并观察其迟发型超敏反应发生的情况。结果：ＤＮＣＢ高免卵黄透析液组、对照卵黄透析液组和生理盐水组的皮肤反应（Ａ值）分别为０．０５２±０．０２２、０．０２８±０．０１４和０．０３２±０．０１２（Ｐ＜０．０５）。结论：高兔卵黄透析液含有某些介导细胞免疫的小分子物质。Purpose: The aim was to find a new method for preparing specific transfer factor (TF). Methods: The hens were immunized with 1-chloro-2, 4-dinitrochlorobenzene (DNCB) and their delayed type hypersensitivity was observed. Hyperimmune egg yolk dialysates to DNCB were prepared and injected into KM mice's abdominal cavity. Six days later, the mice were challenged with DNCB and delayed type skin hypersensitivity was observed. Results: The skin response (A) of hyperimmune egg yolk dialysates to DNCB group, control egg yolk dialysates group and NS group were 0.052 ± 0. 022, 0. 028 ± 0. 014 and 0. 032 ± 0. 012 respectively (P < 0. 05). Conclusion: Hyperimmune egg yolk dialysates contain certain small molecule substance which can mediate cellular immunoresponse.

关 键 词：卵黄 转移因子 迟发型超敏反应 实验 

分 类 号：R392－33[医药卫生—免疫学]