高效液相色谱法测定日化用品中苏丹红类着色剂  

HPLC Determination of Sudan Red Colorants in Daily-Use Chemical Articles

作　　者：田玉平[1] 武晓剑 

机构地区：[1]上海市计量测试技术研究院,上海201203 [2]上海市日用化学工业研究所,上海200062

出　　处：《理化检验（化学分册）》2011年第7期811-812,815,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：上海市科学技术委员会资助(09142200901;10142201100)

摘　　要：应用高效液相色谱法测定了日化用品中苏丹红类着色剂(包括苏丹红Ⅰ、Ⅱ、Ⅲ及Ⅳ)的含量。样品用丙酮-乙腈(1+3)混合溶液提取后,经ZORBAX Eclipse XDB-C_8色谱柱分离,以不同体积比的甲酸-水(0.1+99.9)溶液和甲酸-乙腈(0.1+99.9)溶液为流动相梯度洗脱,用二极管阵列检测器检测。苏丹红Ⅰ的检测波长为478nm,苏丹红Ⅱ、Ⅲ的检测波长为500nm,苏丹红Ⅳ的检测波长为520nm。4种苏丹红的线性范围均为0.50～50mg·L^(-1),检出限(3S/N)均为0.05mg·L^(-1)。以空白样品为基体,进行加标回收试验,苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ的回收率在92.0%～102.1%之间。HPLC was applied to the determination Sudan red colorants including SudanⅠ,Ⅱ,ⅢandⅣin daily chemical articles.The sample was extracted with acetone-acetonitrile（1＋3） mixture.The extract was separated on ZORBAX Eclipse XDB-C_8 column by gradient elution with eluants containing different proportions of solution of A[HCOOH-H_2O（0.1＋99.9）]and B[HCOOH-CH_3CN（0.1＋99.9）],and determined with diode array detector at the wavelengths of 478 ran for SudanⅠ,500 nm for SudanⅡandⅢ,520 nm for SudanⅣ. Linearity ranges for the 4 Sudan red colorants were found same in the range of 0.50-50 mg·L^（-1）,with detection limit（3S/N） of 0.05 mg·L^（-1）.Using blank sample as matrix,recovery test was made by standard addition method,values of recovery found were in the range of 92.0%-102.1%.

关 键 词：高效液相色谱法 苏丹红 着色剂 

分 类 号：O652.63[理学—分析化学]