Proteomic profiling of hepatitis B virus-related hepatocellular carcinoma with magnetic bead-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry  

作　　者：Taotao Liu Ruyi Xue Xiaowu Huang Danying Zhang Ling Dong Hao Wu Xizhong Shen 

机构地区：[1]Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai 200032, China [2]Liver Surgery, Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China

出　　处：《Acta Biochimica et Biophysica Sinica》2011年第7期542-550,共9页生物化学与生物物理学报（英文版）

基　　金：This study was supported by the grants from the National Basic Research Program of China （2007CB936000）, Major National Science and Technology Projects （2009ZX10004-301 and 2008ZX10002-017）, Shanghai Science and Technology Commission （10410709400 and 104119 50100）, Shanghai Talent Development Foundation （2009-035）, and National Natural Science Foundation of China （30772505, 30872503 and 81000968）.

摘　　要：Proteomic techniques are promising strategies in the surveillance of hepatocellular carcinoma （HCC）. This study aimed to investigate the serum profiling with magnetic bead （MB） and matrix-assisted laser desorption/ionization time-of-flight （MALDI-TOF） mass spectrometry （MS） and to further identify the biomarkers for HCC. Serum samples from 80 chronic hepatitis B （CHB） patients, 94 HCC concomitant with HBV patients and 24 healthy subjects were examined by MALDI-TOF MS after peptide enrichment on MBs. Based on the genetic algorithm, diagnostic models for HCC were established between 30 HCC patients and 24 healthy subjects/30 CHB patients. Validations were done with the remaining cases. Markers in the models were identified through liquid chromatography （LC）/MS-MS. The three groups were well separated from each other and two discrimination models were established for HCC. The overall recognition capability of these two models was 96.25% and 93.33%, respectively. Validations showed the misdiagnosis ratio for HCC was 1.6% and 23.4%, respectively. The identified biomarkers for HCC included prothrombin precursor （fragment）, calcium-dependent secretion activator 1, Baculoviral inhibitor of apoptosis repeat-containing protein 6, etc. MB-based MALDI-TOF MS is applicable in identifying the serum biomarkers and can be used in the surveillance of HCC among HBV-infected patients.Proteomic techniques are promising strategies in the surveillance of hepatocellular carcinoma （HCC）. This study aimed to investigate the serum profiling with magnetic bead （MB） and matrix-assisted laser desorption/ionization time-of-flight （MALDI-TOF） mass spectrometry （MS） and to further identify the biomarkers for HCC. Serum samples from 80 chronic hepatitis B （CHB） patients, 94 HCC concomitant with HBV patients and 24 healthy subjects were examined by MALDI-TOF MS after peptide enrichment on MBs. Based on the genetic algorithm, diagnostic models for HCC were established between 30 HCC patients and 24 healthy subjects/30 CHB patients. Validations were done with the remaining cases. Markers in the models were identified through liquid chromatography （LC）/MS-MS. The three groups were well separated from each other and two discrimination models were established for HCC. The overall recognition capability of these two models was 96.25% and 93.33%, respectively. Validations showed the misdiagnosis ratio for HCC was 1.6% and 23.4%, respectively. The identified biomarkers for HCC included prothrombin precursor （fragment）, calcium-dependent secretion activator 1, Baculoviral inhibitor of apoptosis repeat-containing protein 6, etc. MB-based MALDI-TOF MS is applicable in identifying the serum biomarkers and can be used in the surveillance of HCC among HBV-infected patients.

关 键 词：BIOMARKER hepatocellular carcinoma hepatitis B virus MALDI-TOF MS 

分 类 号：Q51[生物学—生物化学] O657.63[理学—分析化学]