基因芯片检测难治性癫脑组织中sh3gl2的表达  

The Expression of sh3gl2 in the Brain Tissues of Intractable Epilepsy Monitored with Gene Chip

作　　者：陈颂春[1] 徐翔[1] 张维[1] 刘桂冬[1] 

机构地区：[1]复旦大学附属华东医院神经内科,200040

出　　处：《中国临床神经科学》2011年第4期363-367,共5页Chinese Journal of Clinical Neurosciences

基　　金：复旦大学附属华东医院院级课题(编号:H-463)

摘　　要：目的:运用基因芯片和RT-PCR技术检测难治性癫脑组织中sh3gl2 mRNA表达,从分子水平探讨难治性癫可能的发病机制。方法:在应用基因芯片对难治性癫患者手术切除的颞叶组织与对照组行基因表达谱分析研究的基础上,筛选出目的基因后用RT-PCR对芯片扫描结果进行验证。结果:候选基因sh3gl2在难治性癫患者脑部颞叶组织中出现高表达,与对照组相比差异有显著统计学意义(P<0.01);RT-PCR结果与芯片结果一致。结论:sh3gl2在难治性癫颞叶皮质中的表达增加,提示了其可能是难治性癫发生发展中的一个重要因素。Aim：To investigate the expression of sh3gl2 mRNA in the brain tissues of patients with intractable epilepsy patients using cDNA microarray and reverse transcription polymerase chain reaction（RT-PCR）,to evaluate the possible molecular pathogenesis of intractable epilepsy.Methods：The intractable epilepsy samples and control cases were studied by means of cDNA microarray.Then the candidate genes were selected and confirmed by RT-PCR.Results：The candidate genes of sh3gl2 mRNA were over-expressed in the temporal lobe of intractable epilepsy patients and compared with the control cases.There was statistical significant difference（P0.01）.The results of PCR were coincided well with the cDNA microarray results.Conclusion：The significant increase of the expression of sh3gl2 in the temporal lobe cortex of intractable epilepsy suggested that it might play an important role in the pathogenesis of intractable epilepsy.

关 键 词：基因芯片 癫 sh3gl2基因 

分 类 号：R742.1[医药卫生—神经病学与精神病学]