细胞内氯离子通道蛋白1蛋白表达与结直肠癌发生、发展及预后的关系  

Expression of Chloride Intracellular Channel Protein 1 Protein Correlated with Tumorigenesis,Tumor Progress,and Prognosis of Colorectal Cancer

作　　者：杨小松[1] 池畔[1] 

机构地区：[1]福建医科大学附属协和医院普外科,福建福州350001

出　　处：《中国普外基础与临床杂志》2011年第7期745-749,共5页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨细胞内氯离子通道蛋白1(CLIC1)蛋白在结直肠正常黏膜、腺瘤及癌组织中的差异性表达及其与结直肠癌发生、发展和预后的关系。方法采用免疫组化组织芯片方法检测150例结直肠正常黏膜组织、62例结直肠腺瘤组织以及187例结直肠癌组织中的CLIC1蛋白表达情况,并分析CLIC1蛋白表达与结直肠癌临床病理因素及预后的关系。结果 CLIC1蛋白在结直肠正常黏膜组织(26.00%,39/150)、腺瘤组织(66.13%,41/62)和癌组织(82.89%,155/187)中的表达阳性率依次升高,之间的差异均有统计学意义(P<0.001)。CLIC1蛋白表达与结直肠癌的TNM分期有关(P=0.007),而与患者性别(P=0.553)、年龄(P=0.206)、肿瘤直径(P=0.185)、肿瘤分化程度(P=0.062)及肿瘤部位(P=0.598)均无关。CLIC1蛋白阳性表达者术后中位生存时间为80个月,CLIC1阴性表达者术后中位生存时间为111个月。log-rank检验表明,CLIC1蛋白阳性表达患者生存率(66.40%)明显低于阴性表达者(80.00%),P=0.031。结论 CLIC1蛋白表达与结直肠癌的发生、发展及预后有关,CLIC1可能是一个潜在的肿瘤标志物。Objective To explore the expression of chloride intracellular channel protein 1（CLIC1） protein in the matched colorectal normal mucosa tissue,colorectal adenoma tissue,and colorectal cancer tissue,and its relationship with tumorigenesis,tumor progression,and prognosis of patients with colorectal cancer.Methods The expression of CLIC1 protein was detected in 150 cases of colorectal normal mucosa tissues,62 cases of colorectal adenoma tissues,and 187 cases of colorectal cancer tissues by using immunohistochemistry tissue microarray,and the relationships between the expression of CLIC1 protein and clinicopathologic features,and the survival rate of patients with colorectal cancer were analyzed.Results The positive rate of CLIC1 protein expression in normal mucosa tissues（26.00%,39/150）,colorectal adenoma tissues（66.13%,41/62）,and colorectal cancer tissues（82.89%,187/155） increased in turn and the difference was statistically significant（P〈0.001）.The expression of CLIC1 protein was related to TNM staging（P=0.007）,but it was not related to gender（P=0.553）,age（P=0.206）,tumor diameter（P=0.185）,tumor differentiation（P=0.062）,and tumor location（P=0.598）.The median survival time after surgery in patients with CLIC1 protein positive expression was 80 months,and it was 111 months in patients with CLIC1 protein negative expression.The survival rate of patients with CLIC1 protein positive expression was lower than that with CLIC1 protein negative expression by log-rank test（66.40% vs.80.00%,P=0.031）.Conclusions The expression of CLIC1 protein is related to the tumorigenesis and progression of colorectal cancer as well as the survival of patients with colorectal cancer.CLIC1 is a potential tumor biomarker.

关 键 词：结直肠癌 细胞内氯离子通道蛋白1 免疫组织化学 组织芯片 生存分析 

分 类 号：R735.34[医药卫生—肿瘤]