右旋柠烯对大鼠大肠癌淋巴道转移的影响  

The effect of D-Limonene on lymphatic metastasis in colorectal cancer of rat

作　　者：刘艳翠[1] 牛莹莹[2] 安宁[3] 

机构地区：[1]牡丹江医学院,黑龙江157011 [2]牡丹江医学院公共卫生学院 [3]牡丹江医学院红旗医院

出　　处：《医学动物防制》2011年第7期630-630,633,共2页Journal of Medical Pest Control

摘　　要：目的观察右旋柠烯(D-Limonene)对大鼠大肠癌淋巴结转移及淋巴管生成的影响,为临床大肠癌术后治疗提供理论支持。方法二甲基肼诱发大鼠大肠癌模型40例。14周将大鼠分为治疗组和对照组,前者给予5%D-limonene(色拉油稀释)灌胃,后者给予等量色拉油做阴性对照。28周取材,免疫组化染色LYVE-1标记淋巴管,观察其分布。结果 LYVE-1标记的淋巴管多分布在癌周组织,中心区域未见淋巴管的阳性表达,D-limonene治疗组淋巴结转移率为5%,淋巴管数密度为2.4±0.83,与对照组(40%,6.1±1.35)比较,差异均有统计学意义(P<0.05)。结论 D-limonene减少肿瘤组织淋巴管的生成,降低淋巴道的转移。Objective To observation the impact of D-Limonene on lymphatic metastasis and lymphangiogenesis in colorectal cancer of rat,and provide theoretical for supporting clinical treatment of colorectal cancer patients.Method 40 colorectal cancer model of rat were induced by 1% dimethylhydrazine（DMH）.The rats were divided into treatment group and control group after 14 weeks.The former was given 5% D-limonene（salad oil dilution） ig,while the latter was given contour and salad oil as the negative control.The animals were killed at 28 weeks,the distributions of lymphatic vessel marked by LYVE-1 were observed.Results Most of lymphatic vessel marked by LYVE-1 distributed in the surrounding area of cancer,while the central region had no positive expression of lymphatic vessel.The lymph node metastasis rate and lymphatic vessel density in treatment group were 5% and 2.4±0.83,respectively.Compared with control group（40% and 6.1±1.35）,there were significant differences（P0.05）.Conclusions Tumor lymphangiogenesis and lymphatic metastasis of colorectal cancer were reduced by D-limonene.

关 键 词：右旋柠烯 大肠癌 淋巴管生成 淋巴转移 

分 类 号：R-332[医药卫生]