Identification of the metabolites of biologically active xanthones isolated from Halenia elliptica D.Don by high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry  

作　　者：Ru Feng Jian Gong Shi Xiao Wei Liu Chun Tao Che John H.K. Yeung Yan Wang 

机构地区：[1]Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China [2]Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China [3]School of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT,, Hong Kong SAR, China [4]Department of Pharmacology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, NZ, Hong Kong SAR, China

出　　处：《Chinese Chemical Letters》2011年第7期839-842,共4页中国化学快报（英文版）

基　　金：the supports of the National Natural Science Foundation of China(No.30873115);National Science and Technology Special Projects(Nos.2009zx09301-003-5-1 and 2009zx09301-003-7-1);supported by the analytical center of the Peking branch of Japanese Shimadzu Corporation to provide the LCMS~n-IT-TOF system

摘　　要：Metabolism study has been carried out on 1-hydroxy-2,3,5-trimethoxyxanthone （HM-1） and 1-hydroxy-2,3,4,7- tetramethoxyxanthone （HM-2）, which are two biologically active ingredients isolated from the Tibetan herb, Halenia elliptica D. Don., in rat liver microsomes in vitro. A method of high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry （LCMSn-ESI-IT-TOF） was applied to analyze metabolites of HM-1 and HM-2 on line, and five metabolites were identified containing 1,5-dihydroxy-2,3-dimethoxyxanthone （HM-5）, 1,7-dihydroxy-2,3,4-trimethoxyxanthone （HM-9）, 1,4, 7- trihydroxy-2,3-dimethoxyxanthone （HM-10）, 1,4-dihydroxy-2,3,7-trimethoxyxanthone （HM-11） and 1,2-dilaydroxy-3,4,7- trimethoxyxanthone （HM-12）. Among these metabolites, HM-9, HM-11, and HM-12 were isomers mutually. The results indicated that HM-1 and HM-2 occurred Phase I metabolic reaction of demethylation in rat microsomes in vitro.Metabolism study has been carried out on 1-hydroxy-2,3,5-trimethoxyxanthone （HM-1） and 1-hydroxy-2,3,4,7- tetramethoxyxanthone （HM-2）, which are two biologically active ingredients isolated from the Tibetan herb, Halenia elliptica D. Don., in rat liver microsomes in vitro. A method of high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry （LCMSn-ESI-IT-TOF） was applied to analyze metabolites of HM-1 and HM-2 on line, and five metabolites were identified containing 1,5-dihydroxy-2,3-dimethoxyxanthone （HM-5）, 1,7-dihydroxy-2,3,4-trimethoxyxanthone （HM-9）, 1,4, 7- trihydroxy-2,3-dimethoxyxanthone （HM-10）, 1,4-dihydroxy-2,3,7-trimethoxyxanthone （HM-11） and 1,2-dilaydroxy-3,4,7- trimethoxyxanthone （HM-12）. Among these metabolites, HM-9, HM-11, and HM-12 were isomers mutually. The results indicated that HM-1 and HM-2 occurred Phase I metabolic reaction of demethylation in rat microsomes in vitro.

关 键 词：Halenia elliptica XANTHONE BIOTRANSFORMATION LCMSn-ESI-IT-TOF Metabolite identification 

分 类 号：O657.63[理学—分析化学] Q949.32[理学—化学]