体外APC/C活性体系的建立及其影响因素的研究  

Preparation of Extracts with APC/C Activity and Analysis of Its Influencing Factors

作　　者：高彦飞[1] 李腾[1] 常艳[1] 王玉博 穆蕊[1] 陈亮[1] 甄诚[1] 韩秋影[1] 于鸣[2] 巩伟丽[1] 张维娜[1] 李慧艳[1] 

机构地区：[1]国家生物医学分析中心,北京100850 [2]军事医学科学院基础医学研究所细胞免疫学教研室,北京100850

出　　处：《科学技术与工程》2011年第21期5154-5157,共4页Science Technology and Engineering

基　　金：国家自然科学基金(30871234)资助

摘　　要：为获得具有细胞周期后期促进复合物(APC/C)活性的细胞裂解液并建立APC/C体外活性检测体系,以其底物Cyc-lin B及Securin的降解为检测指标,分别从细胞阻滞于有丝分裂期的时间,UBCH10(APC/C的E2酶)的浓度以及裂解液浓度等几个方面研究了各种处理因素对APC/C活性的影响,并且用体外翻译底物验证了其活性及相关结论。此项工作为进一步探索APC/C相关分子事件奠定了基础。To prepare the cell extracts with the APC/C activity and analysis of its influencing factors,the degradation of APC/C＇s substrates Cyclin B and Securin was used as a marker.Several assays were done to analysis its influencing factors,including the duration of cells arrest in mitosis,the activity of extracts,the presence of its E2 enzyme,UBCH10,and the concentration of extracts.The similar result was obtained using the in vitro translated substrates.Taken together,these assays were the basement to further study the related molecular mechanism.

关 键 词：后期促进复合物 CYCLIN B SECURIN UBCH10 活性 

分 类 号：Q253[生物学—细胞生物学]