靶向性启动子携带报告基因NIS介导提高胶质瘤摄碘能力  

Increased uptake of radioiodine by glioma cells transfected with human sodium/iodide symporter gene under targeting promoter

作　　者：李承霞[1] 李玮[1] 谭建[1] 

机构地区：[1]天津医科大学总医院核医学科,天津300052

出　　处：《生物医学工程与临床》2011年第4期374-376,共3页Biomedical Engineering and Clinical Medicine

基　　金：天津市应用基础及前沿技术研究计划项目(08JCZDJC23900)

摘　　要：目的在细胞实验中探讨靶向性人端粒酶逆转录酶(hTERT)启动子携带人类钠碘转运体(hNIS)基因能否提高胶质瘤的摄碘能力。方法检测在hTERT启动子引导下表达hNIS基因的腺病毒,使用该腺病毒转染U87和U251细胞系,然后使用γ记数仪检测转染后细胞的摄碘能力。结果实验组肿瘤细胞在转染Ad-hTERT-hNIS后,相对于对照组细胞摄碘率提高约30～40倍。结论实验证明hTERT启动子能引导胶质瘤细胞系表现出肿瘤特异性的碘摄取。Objective To investigate the effect of human sodium/iodide symporter（hNIS） gene under human telomerase reverse transcriptase（hTERT） promoter on the radioiodine uptake by glioma cells.Methods The recombinant adenovirus expressing hNIS gene under hTERT promoter was used to transfect the U87 and U251 malignant glioma cell lines.The radioiodine uptake by the cells was detected using γ-counter.Results The iodide uptake by Ad-hTERT-hNIS transfected cells was about 30-40 times higher than that by the control cells.Conclusion It is demonstrated that the tumor-specific iodide uptake has been achieved by the hTERT promoter-directed hNIS expression in malignant glioma cell lines.

关 键 词：胶质瘤 NIS基因 人端粒酶逆转录酶启动子 放射性碘 

分 类 号：R739.41[医药卫生—肿瘤]