EGCG经AKT1-Mdm-2-p53途径抑制卵巢癌HO-8910细胞的增殖  

Epigallocatechin-3-gallate Inhibits Proliferation of Ovarian Cancer HO-8910 Cells by AKT1-Mdm-2-p53 Pathway

作　　者：李维[1,2,3] 罗瞳[4] 罗招阳[1,2] 

机构地区：[1]南华大学医学院肿瘤研究所,湖南衡阳421001 [2]南华大学医学院病理学教研室,湖南衡阳421001 [3]湖南浏阳市人民医院妇科,湖南浏阳410300 [4]湖南浏阳市人民医院烧伤科,湖南浏阳410300

出　　处：《现代生物医学进展》2011年第15期2840-2843,共4页Progress in Modern Biomedicine

摘　　要：目的:初步探讨EGCG对卵巢癌HO-8910细胞增殖的抑制作用及其机制。方法:通过绘制细胞生长曲线、平皿克隆和软琼脂集落形成实验观察EGCG对HO-8910细胞增殖的抑制作用;Western-blotting检测AKT1、Mdm-2与p53蛋白的表达。结果:(1)细胞生长曲线、平皿克隆和软琼脂集落形成实验结果显示,EGCG可有效抑制HO-8910细胞的增殖(n=3,P<0.05)。(2)Westernblotting检测结果显示,EGCG处理后AKT1与Mdm-2蛋白表达均降低,而p53蛋白表达升高(P<0.05)。结论:EGCG通过抑制HO-8910细胞中AKT1与Mdm-2蛋白表达,促使p53蛋白表达而发挥其对细胞增殖的抑制作用。Objective： To investigate mechanism of Epigallocatechin-3-gallate （EGCG） inhibited the proliferation of ovarian cancer HO-8910 cells. Methods： EGCG inhibited HO-8910 cellular proliferation was observed by the drawing cellular growth curve, plate colony formation and soft agar colony formation. The expressions of AKT1, Mdm-2 and p53 proteins were detected by Western-blotting. Results： 1. The results of cellular growth curve, plate colony formation and soft agar colony formation showed that EGCG active depressed the proliferation of HO-8910 cells with treatment time extended （n=3, P 〈 0.05）. 2. The western-blotting results exhibited that the expressions of AKT1 and Mdm-2 proteins in HO-8910 cells obviously decreased, but the expression of p53 protein increased after EGCG dealing with it （P 〈 0.05）. Conclusion： EGCG depressed the expressions of AKT1, Mdm-2 proteins in HO-8910 cells and increased the expression of p53 protein to inhibit the cellular proliferation.

关 键 词：EGCG 卵巢癌 HO-8910细胞 AKT1蛋白 Mdm-2蛋白 P53蛋白 

分 类 号：R737.9[医药卫生—肿瘤]