6个黄牛群体MyoG基因单核苷酸多态性及其与体尺性状的相关性  

Single nucleotide polymorphism of MyoG gene in six cattle populations and its association with body measurement traits

作　　者：薛梅[1] 昝林森[1,2] 王洪宝[1,2] 高丽[1] 宋付标[1] 王洪程[1] 焦阳[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100 [2]国家肉牛改良中心,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2011年第7期35-42,共8页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家肉牛牦牛产业技术体系建设专项项目(CARS-38);国家转基因生物新品种培育科技重大专项项目(2008ZX08007-002);教育部长江学者与创新团队发展支持计划项目(IRT0940);陕西省"13115"科技创新工程专项项目(2010ZDGC-01)

摘　　要：【目的】研究牛肌细胞生成素基因（MyoG）第2、3外显子及其侧翼区单核苷酸的多态性（SNPs）,分析其与牛部分体尺性状的相关性。【方法】以6个牛群体（鲁西牛、鲁杂牛（鲁西牛×西门塔尔牛）、南阳牛、夏南牛、郏县红牛、秦川牛）共779头3-4岁左右母牛为研究材料,通过PCR-SSCP和DNA测序技术检测牛MyoG基因上存在的SNPs,并分析其与牛部分体尺性状的关联性。【结果】MyoG基因第2外显子及其侧翼区的扩增片段中不存在多态性,第3外显子及其侧翼区的扩增片段中存在3种基因型（AA、AB、BB）,测序结果表明该突变位于3-′UTR 2 109位点处。最小二乘法分析表明,各群体中AA基因型个体的体斜长均显著高于BB基因型个体（P〈0.05）;鲁西牛群体中AA基因型个体的尻长显著高于BB基因型（P〈0.05）;郏县红牛群体中AA基因型个体的体高显著高于BB基因型（P〈0.05）。3种基因型对其他体尺性状没有显著影响（P〉0.05）。【结论】MyoG基因对黄牛体尺性状有一定影响,可作为黄牛体尺性状标记辅助选择的候选基因。【Objective】 This research was to study the polymorphism in exon 2 and 3 and their flanking regions of MyoG gene,and to analyze its relationship with several body measurement traits of cattle.【Method】 779 female individuals aged 3-4 years from six populations（Luxi,Luxi×Simmental crossbred steers,Nanyang,Xia＇nan,Jiaxian red and Qinchuan） were selected to detect polymorphism of MyoG gene by using PCR-SSCP and DNA sequencing technique,and to study its association with part body measurement traits.【Result】 The results indicated that there was not polymorphism in exon 2 and its flanking region and there were three genotypes（AA,AB,BB） detected by exon 3 and its flanking region.The sequencing results showed that the mutation of AG was detected in 3′-UTR of the bovine MyoG gene.Statistical analysis indicated that in all populations,animals with AA genotype have higher mean values for body length than those with the BB genotype（P0.05）;in Luxi population,animals with the AA genotype have higher mean values for rump length than those with the genotype BB（P0.05）;in Jiaxian red population,animals with the AA genotype have higher mean values for body height than those with the BB genotype（P0.05）.Genotype analysis did not show any significant effect on other traits（P0.05）.【Conclusion】 It was concluded that MyoG gene has some effect on body measurement traits and can be used as a candidate gene for marker-assisted selection in cattle.

关 键 词：黄牛 MYOG基因 PCR-SSCP 体尺性状 

分 类 号：S823.811.2[农业科学—畜牧学]