小麦品种面粉色泽相关PPO基因的多态性分析  

Polymorphism Analysis of PPO Genes Related to Flour Color of Wheat

作　　者：王欣[1] 窦秉德[1] 叶建[1] 董红梅[1] 孙美娟[1] 马跃[1] 冯雪娇[1] 朱晓滨[1] 童婷[1] 张瑞越[1] 顾正中[2] 高爱农[3] 

机构地区：[1]淮阴师范学院植物生物技术研究所/江苏省环洪泽湖生态农业生物技术重点实验室,淮安223300 [2]江苏省农业科学院徐淮地区淮阴农科所,淮安223300 [3]中国农业科学院作物科学研究所,北京100081

出　　处：《植物遗传资源学报》2011年第4期594-600,共7页Journal of Plant Genetic Resources

基　　金：江苏省大学生创新实践项目(2008);国家自然科学基金(30771380)

摘　　要：以261个小麦品种(系)组成原始群体,利用其多态性分子标记信息,通过模拟核心种质抽提的方法精选了100个品种(系),对入选种质进行了群体遗传结构分析,并对属于3个亚群100个品种(系)的PPO基因的等位变异进行了检测,分析发现100个小麦品种(系)中Ppo-A1 a、Ppo-A1 b、Ppo-D1 a和Ppo-D1 b的基因频率分别为43%、57%、72%、28%,为小麦PPO活性的分子标记辅助选择(MAS)提供了基础资料。To obtain desirable hybrid parents for improving the quality of wheat flour color,261 wheat cultivar lines from our country′s gene pool were selected as a original population and their genetic polymorphism were analyzed based on molecular markers in wheat genome.A proposed core germplasm group of wheat lines was constructed which included 100 cultivar lines,the proposed core germplasm group was divided into three sub-groups by population genetic structure analysis.The allele diversity at two PPO gene loci were checked in 100 lines of the proposed core gernplasm group,the results showed that the frequencies of Ppo-A1a 、Ppo-A1b、Ppo-D1a and Ppo-D1b were 43%、57%、72%、and 28% in the 100 cultivars lines respectively.The data has supplied a basic information for improvement of wheat PPO activity in the marker-assisted selection.

关 键 词：小麦 面粉色泽 PPO基因 多态性分析 

分 类 号：S512.1[农业科学—作物学]