用聚合酶链反应检测结肠癌人乳头状瘤病毒基因  

Detection of human papillomavirus gene in biopsies from colon carcinoma by PCR

作　　者：朱秋屏[1] 曹建彪[2] 李世荣[2] 谷淑燕[1] 

机构地区：[1]中国预防医学科学院病毒学研究所,北京100052 [2]北京军区总医院消化科

出　　处：《中华实验和临床病毒学杂志》1999年第4期352-354,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　应用聚合酶链反应,检测人乳头状瘤病毒(Humanpapillomavirus,HPV)基因与结肠癌及癌区周边组织的相关性。方法　将结肠镜检获取的72例活检标本进行病理检测,其中结肠癌46例,非癌26例(结肠癌周边组织标本10例),标本用蜡块包埋与固定液两种方法固定,用聚合酶链反应(PCR)特定DNA片段体外扩增法。结果　结、直肠癌人乳头状瘤毒基因检测阳性率434%,结、直肠癌周边组织阳性率10%,非癌组织阳性率为0%。结论　癌区组织基因(HPVs)检测率较高,与非癌对照组相比,差异有显著性(P<0.05),癌组织中HPVs主要以HPV1633型和HPV18型为主,统计学分析表明结、直肠癌的发生、发展与HPV感染有一定的相关性,尤以HPV16型关系更为密切。Objective To detect the human papillomavirus gene (HPVs) in biopsies from colon carcinoma by PCR and to study the relationship between HPV infection and colon carcinoma.Methods 46 biopsies from colon carcinoma, 10 biopsies from tissue around carcinoma and 16 control biopsies were collected and DNA were extracted. The HPV DNA in biopsies were amplified by PCR using general primers (GP) and primers for HPV6/11, HPV16/33 and HPV18.Results 20 out of 46 biopsies from colon carcinoma showed HPV gene positive, HPV6/11, HPV6/33 and HPV18 positivity were 3、11 and 6 respectively. HPV DNA was found in 1 case from 10 biopsies of surroun ding tissue of carcinoma. 16 control biopsies were negative.Conclusion HPV may be related to the etiology of colon carcinoma.

关 键 词：乳头状瘤病毒 人 结肠直肠肿瘤 聚合酶链反应 基因 

分 类 号：R735.350.2[医药卫生—肿瘤] R373.9[医药卫生—临床医学]