中空纤维膜细胞培养器微生物培养试验观察  

OBSERVATION OF MICROBE CULTURING EXPERIMENT IN CELL CULTURE REACTOR OF HOLLOW FIBER MEMBRANES

作　　者：张丽云[1] 王晓光[1] 鲁春起 宋振强[2] 魏建敏[3] 韩德昌[3] 

机构地区：[1]天津医科大学公共卫生学院营养教研室 [2]天津医科大学第二医院 [3]天津纺织工学院膜分离工程研究所

出　　处：《天津医科大学学报》1999年第4期1-2,共2页Journal of Tianjin Medical University

基　　金：天津市自然科学基金资助项目

摘　　要：目的 :对 3种中空纤维膜细胞培养器 (下将细胞培养器省去 )微生物培养进行实验观察。方法 :1 .对 3种中空纤维膜培养活肠链球菌最佳生长时间的试验及其菌体生长特性比较。 2 .对中空纤维聚砜膜 ( PS)培养活肠链球菌最适生长时间、温度 p H的试验。结果 :1 . 3种不同中空纤维膜培养活肠链球菌最佳生长时间为 1 8h。中空纤维聚砜膜、聚丙烯腈膜 ( PAN)培养活肠链球菌检测牛奶凝固良好 ,中空聚偏氟乙烯膜 ( PVDF)牛奶凝固不完全。2 .中空纤维聚砜膜活肠链球菌最适生长时间为 1 8h,最适生长温度为 35℃～ 37℃ ,最适 p H6.0。结论 :1 .3种中空纤维膜培养微生物是可行的。 2 .活肠链球菌在Objective:To observe microbe culturing experiment in three different kinds of cell culture reactor of hollow fiber membranes (hereinafter called “hollow fiber membranes”) Methods:1 Experiment of livina enterostreptococcus culturing on its best living time in three different hollow fiber membranes and their comparison of cultured thalli growing situation 2 Experiment of living enterostreptococcus culturing on its best living time, temperature, and pH value in polysulfone supper filtrate membrane Results:1 The best living time of living enterostreptococcus in three different hollow fiber membranes is 18 hours In the experiment of living enterostreptococcus culturing in hollow fiber polysulfone(PS), and polyacrylonitrile (PAN) membranes, milk immobilized well, and in hollow polyvinylinglidera difluvride (PVDF) membrane, milk immobilized incompletely 2 The best living temperature in polysulfone filtrate membrane is 35~37℃, and pH value is pH=6 0 Conclusion:1 The way of microbe culturing in three different kinds of hollow fiber membranes is workable 2 The living condition of living enterostreptococcus in three different hollow fiber membranes is as same as pharmacopoeia's requirement

关 键 词：中空纤维膜 培养器 微生物 培养 活肠链球菌 

分 类 号：Q93－335[生物学—微生物学]