FISH杂交前与杂交后染色体G显带  

The G-banding before and after Fluorescence in situ hybridization

作　　者：夏薇[1] 崔新羽[1] 苗晓辉[1] 张亚丽 

机构地区：[1]吉林医学院血液检验教研室

出　　处：《吉林医学院学报》1999年第4期21-22,共2页

摘　　要：目的；比较杂交前，后杂交及Ｇ显带结果，探讨ＦＩＳＨ染色体Ｇ显带最佳方法。方法：参照Ｇａｒｓｏｎ等Ｇ显带法略作改进作杂交前Ｇ显带。然后杂交。参照Ｐｏｐｅｓｃｕ方法略作改进进行杂交后Ｇ显带，将二种结果进行对比。结果；杂交前Ｇ显带法优于杂交后Ｇ显带。结论：经改进后的先进行Ｇ显带，再进行杂交方法可较好地用于ＦＩＳＨ和Ｇ显带技术中。Objective:To compare the result of the G-banding before FISH and the G-banding after FISH,to study which methods is better.Methods:To hybridize according to the method of Garson's G-banded chromosomes and to make G-band according to Popescu's methods after hybridization.The two results were compared then.Result:The G-banding before Fluorescence in situ hybridization is better than the method after FISH.Conclusion:The improved methods of G-bandind before Fluorescence in situ hybridization can give satisfied FISH signals and G-banding.

关 键 词：G显带 荧光原位杂交 染色体 

分 类 号：R394[医药卫生—医学遗传学]