油橄榄叶中橄榄苦苷的分离纯化  被引量:7

Separation and purification of oleuropein from olive leaves

在线阅读下载全文

作  者:傅珊[1] 万文倩[1] 杨文革[1] 胡永红[1] 

机构地区:[1]南京工业大学生物与制药工程学院,南京210009

出  处:《生物加工过程》2011年第4期31-34,共4页Chinese Journal of Bioprocess Engineering

基  金:江苏省高校自然科学基础研究项目(08KJA530002)

摘  要:考察了6种不同大孔树脂对橄榄苦苷的吸附,筛选出吸附选择性好的D101大孔吸附树脂为实验材料。得到的D101大孔树脂的最佳吸附条件:试样上样质量浓度41.06 mg/mL,流速5 mL/min,50%乙醇溶液洗脱,洗脱液用量260 mL,树脂可以反复使用。经大孔树脂富集后,橄榄苦苷的纯度可达55%以上,再经葡聚糖凝胶精纯化后,可达76%以上。Extraction, purification and separation of oleuropein in olive leaf extract were studied by 6 different macroporns resins. Macroporous absorption resins were compared on adsorption and desorption performances. To achieve an optimal purification of oleuropein, the adsorption and desorption characteristics of the best resin were investigated. The optimal column separation conditions of macroporous resin D101 were as follows: the mass concentration of oleuropein in the liquid was 41.06 mg/mL, the flow rate was 5 mL/min, 50% aqueous ethanol solution was effective solvent for oleuropein desorption, the volume of eluent was 260 mL. The content of oleuropein was more than 55% , the oleuropein was obtained by purification again using Sephadex, and 76% yield was obtained.

关 键 词:柱层析法 大孔吸附树脂 橄榄苦苷 

分 类 号:R284.2[医药卫生—中药学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象