渗透压对马铃薯种质试管保存的影响效应  

Effect of Osmotic Pressure on Conservation in vitro in Potato Germplasm

作　　者：齐恩芳[1] 王一航[1] 李高峰[1] 贾小霞[1] 文国宏 

机构地区：[1]甘肃省农科院马铃薯研究所,兰州730070

出　　处：《长江蔬菜》2011年第14期20-23,共4页Journal of Changjiang Vegetables

基　　金：国家马铃薯产业技术体系(CARS-10)建设专项资金资助

摘　　要：用添加不同浓度及不同配比蔗糖与甘露醇的培养基对马铃薯试管苗进行离体保存研究。研究结果表明,MS+60 g/L蔗糖+10(20)g/L甘露醇和MS+80 g/L蔗糖10(20)g/L甘露醇均能使马铃薯试管苗连续保存9个月,其中80 g/L蔗糖和20 g/L甘露醇组合处理保存9个月时存活率最高,达到83.3%,且诱导试管薯形成。In vitro conservation of potato in substrate by adding different concentrations of sucrose and mannitol were studied. The results showed that MS substrate supplemented with 60 g/L sucrose ＋ 10 or 20 g/L mannitol and 80 g/L sucrose ＋ 10 or 20 g/L mannitol could prolong seedlings＇ conservation time to 9 months. Especially, 80 g/L sucrose ＋ 20 g/L mannitol bad higher survival rate of 83.3% and the treatment had also induced minitubers.

关 键 词：马铃薯 试管保存 蔗糖 甘露醇 

分 类 号：S532[农业科学—作物学]