脑微血管内皮细胞缺氧复给氧损伤后内皮细胞凋亡的实验研究  

The effects of cAMP on BBB damage in brain injury of rats

作　　者：李良平[1] 俞方毅[1] 许智蕾[1] 陈家祥[1] 徐如祥[2] 

机构地区：[1]广州市红十字会医院,广东广州510220 [2]北京军区总医院

出　　处：《中外医学研究》2011年第22期12-14,共3页CHINESE AND FOREIGN MEDICAL RESEARCH

摘　　要：目的探讨缺氧复给氧损伤后脑微血管内皮细胞凋亡的机制。方法脑微血管内皮细胞缺氧复给氧损伤后,采用流式细胞仪检测细胞凋亡的程度。结果应用毛喉素提高细胞内cAMP和蛋白激酶C抑制剂H7,能明显抑制细胞凋亡。结论细胞凋亡的主要原因是细胞内毛喉素减少和PKC功能增强。Objective To explore the mechanism of apoptosis after hypoxia-reperfusion oxygen damage the cerebral microvascular endothelial cells.Methods Apoptosis was detected with flow cytometry after hypoxia-reperfusion oxygen damage of the cerebral microvascular endothelial cells.Results Forskolin, the reagent of elevating cellular content of cAMP and H7,a PKC inhibitor can significantly inhibit apoptosis.Conclusion The main reasons of apoptosis are the cAMP decrease and PKC increase in cells.

关 键 词：内皮细胞 缺氧复给氧 细胞凋亡 

分 类 号：R743[医药卫生—神经病学与精神病学]