茶树幼苗发育过程中儿茶素合成与积累变化的研究  

Catechins synthesis and accumulation in tea seedlings at different development stages

作　　者：单育[1] 李伟伟[2] 王云生[2] 刘亚军[1] 王弘雪[1] 王晓帆[1] 卢忠尉[2] 田艳维[2] 高丽萍[1,2] 夏涛[1] 

机构地区：[1]安徽农业大学教育部茶叶生物化学与生物技术重点实验室,合肥230036 [2]安徽农业大学生命科学学院,合肥230036

出　　处：《安徽农业大学学报》2011年第4期600-605,共6页Journal of Anhui Agricultural University

基　　金：973计划前期项目(2007CB116211);国家自然科学基金(30771755;30972401和31000314);安徽省自然科学基金(09041-1006);安徽省教育厅自然科学研究项目(KJ2010A117)共同资助

摘　　要：以茶树幼苗为材料,以HPLC、qRT-PCR为分析手段,分析了茶树幼苗发育过程中根茎叶的儿茶素含量变化及相关合成基因的表达差异。结果显示,在发育过程中,不同器官中儿茶素总量呈现不同的变化规律,在幼苗发育25 d、45 d、75 d和90 d时,茶树叶片的儿茶素含量(干重)呈下降趋势,分别为79.99、57.69、49.29和33.26 mg.g-1;茎中(干重)的为14.40、11.89、8.54和9.29 mg.g-1;根中的含量(干重)很低,分别为1.03、0.60、0.82和1.62 mg.g-1;在发育25 d的茶树幼苗茎叶中6种儿茶素单体均可以检测到;根中缺乏酯型儿茶素,但在发育10 d的根中却可以检测到。在茶树幼苗不同器官中,酚类物质合成相关基因表达有较大的差异性。The tea seedlings at different development stages were taken as experimental materials,and the contents of catechins and the expressions of genes related to catechin synthesis in tea leaves,stems and roots of the tea seedlings at different development stages were analyzed using HPLC and qRT-PCR techniques.During the tea seedling development stages,we find that the content of catechins in different organs show different trends.The content of catechins in leaves（DW） were 79.99,57.69,49.29 and 33.26 mg.g-1 when the seedlings growing for 25 days,45 days,75 days and 90 days,showing a downtrend;while the content of catechins（DW） were 14.40,11.89,8.54 and 9.29 mg.g-1 in stems and the content of catechins（DW） were 1.03,0.60,0.82 and 1.62 mg.g-1 in roots.We can find six kinds of catechins in 25 day＇s tea seedlings and can not found ester type catechins in tea roots except for in 10 day＇s seedling.Expressions of the related genes involved in phenylpropanoid biosynthetic pathway showed remarkable difference in different organs at different growth stages.

关 键 词：茶树 幼苗发育 儿茶素合成 基因表达差异 

分 类 号：S571.1[农业科学—茶叶生产加工]