藏药柳茶提取物调节肥胖大鼠脂代谢的作用机制研究  

Effects and Mechanism of Sibiraea angustata on Lipid Metabolism in Hight-fatted Rats

作　　者：夏苗[1] 王晶宇[1] 马晓燕[1] 卢岩松[2] 田青山[2] 刘昕[1] 

机构地区：[1]兰州大学基础医学院病理与病理生理教研所,甘肃兰州730000 [2]青海省人民医院普外科,青海西宁810007

出　　处：《中药材》2011年第6期922-926,共5页Journal of Chinese Medicinal Materials

基　　金：甘肃省中医药管理局科研项目(GZK-2008-20)

摘　　要：目的:探讨藏药柳茶调节单纯性肥胖大鼠脂质代谢的作用机制。方法:复制单纯性肥胖大鼠模型,以藏药柳茶提取物高、中、低不同剂量灌胃给药8 w后,剥离肾周脂肪制作组织切片,显微镜下观察脂肪细胞数量及形态。实时荧光定量RT-PCR法检测脂肪组织中脂联素(Adiponectin)、脂联素受体2(AdipoR2)、腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)、过氧化物酶体增殖物激活受体γ(Peroxisome Proliferator-activated Recep-torsγ,PPARγ)mRNA水平变化。用Western Blot检测脂肪组织AMPK蛋白表达情况。结果:与模型组比较,使用柳茶提取物8 w后400倍显微镜下全视野范围内脂肪细胞直径减小、数量增加。脂肪组织中Adiponectin、AdipoR2、AMPK、PPARγmRNA水平均有不同程度升高。柳茶中剂量组大鼠脂肪组织AMPK蛋白表达量显著高于模型组。结论:藏药柳茶的减肥作用机制可能是通过脂联素的信号转导途径来完成的。Objective：To investigate the effect and mechanism of Sibiraea angustata on lipid metabolism in hight-fatted SD rats.Methods：After the obese model was built,Sibiraea angustata was administrated intragastrically to obese rats for 8 weeks.Peeled off fat around kidneys and made pathological tissue sections.The number and size of adipocytes were detected.The levels of adiponectin,adipoR2,AMPK,and PPARγ mRNA in adipose tissue were detected by RT-PCR.AMPK protein expression in adipose tissue were detected by Western Blot.Results：Compared with the model group,the diameter of adipocytes were reduced while the number increased after adminiseration of Sibiraea angustata for 8 weeks.The levels of adiponectin,adipoR2,AMPK and PPARγ mRNA were increased siginficantly.The expression of AMPK protein was also up-regulated significantly.Conclusion：Sibiraea angustata has anti-obesity effect.The mechanism may be related to the adiponectin signal transduction pathway.

关 键 词：柳茶 脂联素 脂联素受体2 腺苷酸活化蛋白激酶 过氧化物酶体增殖物激活受体Γ 

分 类 号：R285.5[医药卫生—中药学]