尼克酰胺对小鼠前脂肪细胞增殖及分化影响  

Effects of niacinamide on proliferation and differentiation of 3T3-L1 preadipocytes

作　　者：陈思凡[1] 肖新才[2] 郑琳[1] 孙延双[1] 张子丽[1] 冯翔[1] 

机构地区：[1]中山大学公共卫生学院营养学系,广东广州510089 [2]广州市疾病预防控制中心

出　　处：《中国公共卫生》2011年第8期1014-1015,共2页Chinese Journal of Public Health

摘　　要：目的探讨尼克酰胺(NAM)对小鼠3T3-L1前脂肪细胞增殖及分化的影响及其机制。方法培养3T3-L1前脂肪细胞,添加NAM,观察其对细胞增殖及分化的影响,以添加白藜芦醇(Res)作阳性对照,以不添加任何药物为空白对照;用水溶性四氮唑(WST-1)法检测细胞的增殖,用染色比色法检测细胞的分化,采用蛋白免疫印记(westernblot)技术检测沉默信息调节因子1(Sirt1)、过氧化物酶增殖物激活受体γ(PPARγ)、CCAAT/增强子结合蛋白α(C/EBPα)位点的蛋白质表达。结果干预48 h后,NAM组细胞相对数量是对照组的1.152倍,NAM+Res组是Res组的1.272倍;NAM组细胞相对脂肪是对照组的1.519倍,NAM+Res组是Res的1.321倍;添加NAM后Sirt1、PPARγ、42和24KDa的C/EBPα蛋白表达水平分别是对照组的0.381、2.107、1.005和1.233倍,NAM+Res组Sirt1、PPARγ、42KDa和24KDa的C/EBPα表达水平分别是Res组的0.421、2.226、2.031和1.544倍。结论 NAM可增加3T3-L1前脂肪细胞的增殖和分化,机制可能是通过抑制Sirt1表达而增加脂肪细胞分化相关蛋白PPARγ、C/EBPα的表达。Objective To explore the effects of niacinamide（NAM） on proliferation and differentiation of murine 3T3-L1 preadipocytes.Methods 3T3-L1 preadipocytes were cultured and treated with NAM and resveratrol（Res） in different dosages.Cell proliferation was analyzed with water soluble tetrazole（WST）-1 method.Oil red O staining method and spectrophotography were applied to analyze the degree of differentiation.Western blot was applied to detect the expression of silent information regulator 1（Sirt1）,peroxisome proliferator activated receptor γ（PPARγ）,and CCTTA enhancer binding protein α（C/EBPα）.Results After treated with NAM for 48 hours,compared with the control with or without Res treatment,the relative cell amount increased by 1.152 and 1.272 times and the relative fat content was 1.519 and 1.321 times;the protein levels of Sirt1,PPARγ,42KDa and 24KDa C/EBP α of NAM-treated and NAM＋Res-treated groups were 0.381,2.107,1.005,and 1.233 times and 0.421,2.226,2.031,and 1.544 times compared to those of control group.Conclusion NAM can enhance the proliferation and differentiation of 3T3-L1 preadipocytes,and the underlying mechanisms may include inhibiting expression of Sirt1 and increasing expression of PPARγ and C/EBPα.

关 键 词：尼克酰胺 脂肪细胞 沉默信息调节因子1 过氧化物酶增殖物激活受体Γ CCAAT/增强子结合蛋白α 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]