液相色谱串联质谱法测定动物源性食品中硝呋烯腙的残留量  

DETERMINATION OF NITROVIN IN ANIMAL PRODUCTS BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY

作　　者：徐彦辉[1,2] 何俊[1,2] 顾亮[1,2] 沈宏林[1,2] 陈戈[1,2] 丁磊[1,2] 蒋俊树[1,2] 

机构地区：[1]国家农副加工食品质量监督检验中心 [2]安徽国家农业标准化与监测中心,合肥230051

出　　处：《化学分析计量》2011年第4期29-32,共4页Chemical Analysis And Meterage

基　　金：国家质检总局公益性科研计划项目(2009QK188)

摘　　要：利用液相色谱-串联质谱(LC-ESI-MS/MS)测定动物源性食品中的硝呋烯腙残留量。通过实验,对样品前处理及仪器检测条件进行了优化。样品经提取和固相萃取净化后,采用HPLC-ESI-MS/MS进行检测,在多反应监测模式(MRM)下,外标法定量。方法的检出限为2.0μg/kg,在1.0～100.0μg/L范围内线性关系良好(相关系数r>0.99),平均添加回收率为66%～82%。A new method for determining nitrovin in animal products by liquid chromatography - electrospray ionization tandem mass spectrometry was developed. Sample preparation and HPLC - MS/MS analytical conditions were examined and optimized. Samples were extracted, cleaned up and then detected by LC - ESI - MS/MS method. External standard method was used for quantifi- ID cation in the multiple - reaction monitoring mode. Limit of detection （LOD） was 2.0 μg/kg. The method showed good linearity in the range of 1.0 - 100.0 μg/L for nitrovin （r 〉 0.99）. The average recoveries of nitrovin in animal products ranged from 66% to 82%.

关 键 词：动物源性食品 硝呋烯腙 液相色谱串联质谱 

分 类 号：O657.63[理学—分析化学]