幼儿龋和无龋牙菌斑中白色念珠菌临床分离菌株的致龋性  

Cariogenicity of Candida Albicans Isolated From Children With Early Childhood Caries and Caries Free

作　　者：赵玮[1] 邱荣敏[1] 林艳[2] 卢佳璇[1] 余东升[1] 

机构地区：[1]中山大学.口腔医学研究所.附属口腔医院,广东广州510055 [2]江门市中心医院

出　　处：《中华妇幼临床医学杂志（电子版）》2011年第4期235-238,共4页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：广东省自然科学基金项目(8151008901000076);广东省科技计划社会发展项目(00316971120302088)~~

摘　　要：目的探讨幼儿龋（earlychildhoodcaries，ECC）和无龋（caries-free，CF）牙菌斑中白色念珠菌临床分离菌株导致幼儿龋的差异性。方法将前期研究中保存的3～6岁幼儿牙菌斑白色念珠菌I临床分离菌株，其中来源于幼儿龋牙菌斑为26株（dmft≥1），纳人ECC组；来源于幼儿无龋牙菌斑为24株（dmft=O），纳入CF组。将两组菌株分别接种至不同pH值的葡萄糖沙氏液体培养基中，37℃培养48h后，测定上清液终末pH值以评价产酸能力；分别将两组菌株沉淀用PBS液重悬，采用紫外分光光度仪测定波长540nm处的吸光度（absorbance540，As40）值以评价其耐酸能力。同时将菌液分别接种至羟基磷灰石表面，37℃培养48h后，对黏附至羟基磷灰石表面白色念珠菌进行平板培养，采用菌落形成单位计数法评定其黏附能力。结果当葡萄糖沙氏液体培养基初始pH值≥4．5时，ECC组和CF组白色念珠菌株的产酸和菌株生长能力比较，差异无显著意义（P〉0．05）；当初始pH值为4．0时，ECC组的产酸和生长能力较CF组强，两组比较，差异有显著意义（P〈0．05）。ECC组和CF组白色念珠菌的黏附能力比较，差异无显著意义（P〉0．05）。结论幼儿龋和无龋牙菌斑中白色念珠菌临床分离菌株致龋能力的差异，主要体现在耐酸能力方面。强耐酸性白色念珠菌临床分离菌株可能起到促进幼儿龋齿发展的作用。Objective To discuss the difference of cariogenicity of Candida albicans from children with early childhood caries（ECC） and without caries. Methods Twenty-six strains isolated from children with early childhood caries as the ECC group and 24 strains from children with caries free（CF） as the CF group, were inoculated into Sabouraud liquid medium with different initial pH value （ranging from 4.0 to 7.0 at 0.5 intervals）. After cultured at 37℃ for 48 h, the terminal pH value of medium was detected by pH meter to evaluate the acidogenicity, and the As40 was measured by spectrophotometer to assess the aciduricity. Moreover, all the strains were inoculated into the surface of hydroxyapatite（HA） plate, and colony formation unit was used to evaluate the adhesion ability. The ability of acidogenicity, aciduricity and adhesion of Candida albicans in ECC group and CF group were compared. Results There were no statistically significant difference of the acidogenicity in the changes of pH value and A540 value between ECC and CF group at initial pH value between 4.5-7.0（P〉0.05）, while the change of pH and As40 values of the acidogenicity was significantly higher in ECC group than that of CF group at initial pH value at 4. 0 （P〉0.05）. The difference of adhesion ability between two groups was not statistically significant （P〉0. 05）. Conclusion The difference of Candida albicans from children with early childhood caries and those without caries of cariogenicity; strains of Candida albicans with stronger aciduricity may play an important role in the promoting the process of early childhood caries.

关 键 词：婴幼儿龋 致龋性 产酸性 耐酸性 黏附性 白色念珠菌 

分 类 号：R788.1[医药卫生—口腔医学]