马铃薯块茎组织特异性启动子的克隆及序列分析  

Cloning and Sequencing of Potato Tuber Tissue-specific Promoter Region

作　　者：陈国梁[1] 陈宗礼[1] 贺晓龙[1] 罗茜[1] 

机构地区：[1]延安大学生命科学学院陕西省区域生物资源保育与利用工程技术研究中心,延安716000

出　　处：《生物技术通报》2011年第7期69-72,共4页Biotechnology Bulletin

基　　金：陕西省教育厅省级重点实验室重点科研计划项目(2010JS065);延安市科技发展计划项目(2010kn-03);延安大学专项科研基金项目;延安大学大学生科技创新训练项目(YD2008-106)

摘　　要：利用PCR技术从马铃薯陇薯3号基因组DNA中扩增出长度约为1.0 kb的DNA片段,经与T载体连接,测序表明,克隆到的DNA片段大小为969 bp,该序列与GenBank中已公布的patatin启动子序列同源性为97.94%;采用植物顺式调控元件数据库PLACE和P lantCare进行序列分析,结果表明,该片段含有启动子的保守序列TATA-box和CAAT-box,且在CAAT-box上游有8 bp的增强子。此外,还具有马铃薯块茎蛋白patatin基因启动子保守调控序列的蔗糖效应元件(SURE)4个及马铃薯储藏物特异结合调控patatin蛋白表达位点(B-box)2个,而这些特异序列可能是基因特异表达所必须的。The patatin gene promoter region was amplified from Solanum tuberosumn genomic DNA by polymerase chain reaction（PCR）and cloned into the T-vector.The sequence analysis showed that the size of sequence is 969 bp,and its homology was 97.94% with published sequence of the patatin promoter in GenBank.Using PLACE and PlantCare sequence database analysis showed that the fragment containing the conserved promoter sequence,such as TATA-box,CAAT-box and enhancer element motif upstream of CAAT-box.In addition,the fragment containing four Sucrose Responsive Elements（SURE）of conserved among genes regulated by sucrose in the patatin gene promoter of potato and tow B-boxes of involved in potato tuber-specific and sucrose-inducible gene expression,these specific gene sequences may be necessary for specific expression.

关 键 词：马铃薯 组织特异性 启动子 PCR 

分 类 号：S532[农业科学—作物学] Q943.2[生物学—植物学]