附子对两种不同慢性肾病小鼠乳酸代谢的影响  

The Effects of the Prepared Aconite Root on Lactic Acid Metabolism in Two Kinds of Chronic Renal Diseases Mice Models

作　　者：范建萍[1] 杨金招[1] 王友群[1] 

机构地区：[1]中国药科大学药理研究室,江苏南京210009

出　　处：《药学进展》2011年第7期323-329,共7页Progress in Pharmaceutical Sciences

摘　　要：目的:观察附子对两种不同慢性肾病模型小鼠乳酸代谢的影响,探讨附子的温阳、肾脏保护作用及与乳酸酸中毒的关系。方法:将雄性ICR小鼠随机分为7组:正常对照组、关木通模型组、关木通+附子低剂量组、关木通+附子高剂量组、腺嘌呤模型组、腺嘌呤+附子低剂量组和腺嘌呤+附子高剂量组。正常对照组灌胃等体积饮用水;关木通模型组灌胃给予7 g.kg-1.d-1的关木通水煎剂,给药4天后停药3天,持续5周;腺嘌呤模型组先以250 mg.kg-1腺嘌呤隔天灌胃1次,持续1周,再以130 mg.kg-1腺嘌呤隔天灌胃1次,持续3周;关木通(腺嘌呤)模型+附子低剂量组、关木通(腺嘌呤)模型+附子高剂量组在造模的同时分别给予3、10 g.kg-1.d-1的附子水煎剂。实验结束时,测定各组血清肌酐和尿素氮、乳酸含量及乳酸脱氢酶活性,以及肝、肾、睾丸的蛋白、乳酸含量和乳酸脱氢酶活性,并记录左肾、左睾系数及附睾精子数。结果:附子能显著降低两种肾病模型小鼠的血清尿素氮、乳酸含量、乳酸脱氢酶活性及左肾系数,显著提高精子数及肾脏蛋白含量;显著降低关木通模型小鼠的肝脏乳酸含量、肾脏的乳酸含量和乳酸脱氢酶活性,显著增加肾脏蛋白含量;显著增加腺嘌呤模型小鼠肾脏、睾丸的乳酸含量,提高肝脏、睾丸乳酸脱氢酶活性,降低肾脏乳酸脱氢酶活性及睾丸蛋白含量。附子剂量与药效呈一定的正相关性。结论:两种肾病模型均存在不同程度的乳酸酸中毒。附子对关木通致慢性马兜铃酸肾病的肾脏酸中毒的纠正情况最明显;而腺嘌呤致慢性肾衰模型虽血清乳酸含量显著升高,但肝、肾、睾丸中均未出现乳酸蓄积,相反,肾脏、睾丸中乳酸含量有所降低,附子也能使该模型小鼠的肝、肾乳酸含量和乳酸脱氢酶活性趋于正常值,提示附子的温阳、肾脏保护作用与调节乳酸代谢有关。Object： To observe the effects of the prepared aconite root on lactic acid metabolism in two kinds of mice models with chronic renal diseases including chronic aristolochic acid nephropathy （CAAN） induced by Caulis Aristolochiae Manshuriensis and chronic renal failure（CRF） induced by adenine, and to find out the relationship between wanning yang and kidney-protection effects of the prepared aconite root and lactic acidosis. Methods： Male ICR mice were randomly divided into seven groups including normal control group, model1 （M1） group, model1 ＋ low dose of prepared aconite root（ M1 ＋ FL） group, model1 ＋ high dose of prepared aconite root（ M1 ＋ FH） group, model2（M2） group, model2 ＋ low dose of prepared aconite root （ M2 ＋ FL） group and model2 ＋ high dose of prepared aconite root（ M2 ＋ FH） group. The mice in M1 group and M2 group were administrated Caulis Ariswlochiae Manshuriensis（ 7 g·kg^-1·d^-1, the first four days per week and lasting for five weeks） and adenine （250 mg·kg^-1, every two days in the first week, and 130 mg·kg^-1 every two days in the next three weeks） by gavage, respectively. The mice in M1 ＋ FL group and M1 ＋ FH group were administrated the prepared aconite root 3 and 10 g·kg^-1·d^-1 respectively besides Caulis Aristolochiae Manshuriensis, and the mice in M2 ＋ FL group and M2 ＋ FH group were administrated the prepared aconite root 3 and 10 g·kg^-1·d^-1 respectively besides adenine. At the end of the experiment, the contents of creatine（Cr）, urea nitrogen（BUN）, lactic acid（LD） and the activity of lactate dehydrogenase（LDH） in blood serum, the contents of protein, LD and the activity of LDH in the kidney, li,~er and testis were determined. The kidney, testis coefficient and the number of sperm were recorded as well. Results： The contents of serum BUN, LD, the activity of LDH and mice in both models were significantly reduced, and sperm counts and renal protein kidney coefficient of contents were raised b

关 键 词：附子 慢性马兜铃酸肾病 慢性肾功能衰竭 乳酸 乳酸脱氢酶 

分 类 号：R285.5[医药卫生—中药学]