超声波辅助法提取细叶黄皮叶总黄酮及其清除羟自由基活性的测定  

Ultrasonic-assisted Extraction Technology and Hydroxy Radical Scavenging Activity of Total Flavonoids from the Leaves of Clausena Anisum-olens

作　　者：苏秀芳[1] 郭青[2] 甘海妹[1] 黄智想[1] 

机构地区：[1]广西民族师范学院化学与生物工程系,广西崇左532200 [2]江苏省食品药品检验所,江苏南京210008

出　　处：《化学世界》2011年第7期420-422,431,共4页Chemical World

基　　金：广西自然科学基金项目(2010GXNSFA013050)

摘　　要：对超声波法提取细叶黄皮叶中黄酮类物质进行了研究,通过单因素及L9(34)正交实验,最佳提取工艺条件为A1B3C3D3,即:温度60℃,乙醇浓度60%,超声波功率90 W,料液比1∶25,提取时间70 min。此条件下细叶黄皮叶总黄酮提取率达5.730%。在设置的5个质量浓度梯度下,总黄酮提取液对羟自由基的清除效果与其质量浓度呈正相关,当样品质量浓度为0.694 g/L时,其清除率达49.1%。The ultrasonic-assisted extraction of flavonoids in the leaves of Clausena anisum-olens was studied.The single factor and orthogonal design methods L9（34） were applied for obtaining the optimal technological conditions：temperature at 60 ℃,60 % ethanol,ratio of material to liquid=1∶25,power of 90 W,and extraction time of 70 min.Under the optimal conditions,the extraction percent of total flavonoids was about 5.730 % from the leaves of Clausena anisum-olens.There was a positive correlation between hydroxyl radical scavenging activity and concentration of total flavonoids from the leaves of Clausena anisum-olens.The hydroxyl radical scavenging rate at the concentration level of 0.649 g/L was 49.1%.

关 键 词：细叶黄皮叶 总黄酮 超声波辅助提取 羟自由基 

分 类 号：O657.32[理学—分析化学]