水稻细菌性条斑病菌抑菌植物筛选及杀菌剂活性测定  

Screening of bactericidal plants and determination of inhibitory activity of bactericides against Xanthomonas oryzae pv.oryzicola

作　　者：骆海玉[1] 邓业成[1] 秦卉[1] 钟汉林[1] 李瑞钰[1] 廖永梅[1] 

机构地区：[1]广西师范大学生命科学学院/珍稀濒危动植物生态与环境保护省部共建教育部重点实验室,广西桂林541004

出　　处：《广东农业科学》2011年第11期99-101,共3页Guangdong Agricultural Sciences

基　　金：珍稀濒危动植物生态与环境保护省部共建教育部重点实验室科研项目(桂科能1001Z014);广西自然科学基金(桂科自0991097)

摘　　要：采用带药平板涂布法测定了32种植物甲醇提取物、1种植物精油和13种杀菌剂对水稻细菌性条斑病菌的抑菌活性。结果表明,在植物甲醇提取物中,杠板归、老虎刺、广东蛇葡萄、细叶桉、枫杨提取物的MIC值分别为4 000、6 000、6 000、10 000、10 000 mg/L;其余植物提取物的MIC值>10 000 mg/L。在13种杀菌剂中,64%杀毒矾可湿性粉剂,3%中生菌素可湿性粉剂,58%甲霜.锰锌可湿性粉剂和2%加收米可溶液剂抑菌活性较高,MIC值分别为12.8、15、48.3、80 mg/L;其次为53.8%可杀得水分散粒剂和20%叶枯唑可湿性粉剂,MIC值分别为134.5、400 mg/L;其余杀菌剂的活性较低,20%噻唑锌悬浮剂和72%农用链霉素可溶粉剂的MIC值分别为2 000、4 311.4 mg/L,50%敌克松可湿性粉剂、80%代森锰锌可湿性粉剂、60%甲硫.异菌脲可湿性粉剂、15%三唑酮可湿性粉剂和75%百菌清可湿性粉剂5种药剂在稀释100倍时对水稻细菌性条斑病菌无抑菌活性。Poison plate coating method was used to determine the inhibitory activity of 32 plant methanol extracts, I plant essential oil and 13 bactericides against Xanthomonas oryzae pv. oryzicola. Among the plant methanol extracts, The MICs of Polygonum perfoliatum, Pterolobium punctatum, Ampelopsis cantoniensis, Eucalyptus tereticornis, Pterocarya stenoptera were 4000, 6000, 6000, 10000,10000 mg/L respectively. The MICs of other plants were above 10000 mg/L. Among 13 bactericides, 64% oxadixyl·mancozeb WP, 3% zhongshengmycin WP, 58% metalaxyl. mancozcb WP and 2% kasumin SL showed relatively higb inhibitory activity, with the MIC of 12.8, 15, 48.3, 80 g/L respectively, following by 53.8% copper hydroxide WG and 20% bismerthlazol WP, which showed significant inhibitory activity, with the mininmm inhibitory concentrations （MIC）of 134.5 g/L and 400 g/L respectively. The other bactericides show low inhibitory activity, 20% thiodiazole-Zn WP and 72% agricultural streptomycin had inhibitory activity with concentrations （MIC）of 2000 g/L and 4311.4 g/L respectively; 50% fenaminosulf WP, 80% nmneozeb WP, 60% met · iprodione WP, 15% triadimefon WP and 75% chlorothalonil WP had no inhibitory activities against X. oryzae pv. oryzicola at the concentration of 100 times solution.

关 键 词：水稻细菌性条斑病菌 植物提取物 杀菌剂 抑菌活性 

分 类 号：S435.111.49[农业科学—农业昆虫与害虫防治]