银杏提取物对自身免疫性脑脊髓炎小鼠脱髓鞘免疫炎性病变的影响  

Effects of Ginkgo biloba extract on mice with experimental autoimmune encephalomyelitis

作　　者：陆利[1] 张卫国[1] 杨桂姣[1] 

机构地区：[1]山西医科大学解剖教研室,太原030001

出　　处：《山西医科大学学报》2011年第7期545-547,612,共4页Journal of Shanxi Medical University

基　　金：山西省青年基金资助项目(2009021045);山西省归国留学人员科研项目(2009-50)

摘　　要：目的探讨银杏提取物(GBE)对实验性自身免疫性脑脊髓炎(EAE)小鼠脱髓鞘免疫炎症病变的影响。方法将小鼠分为EAE模型组(腹腔注射生理盐水)和GBE组[腹腔注射GBE 70 mg/(kg.d)],应用髓鞘少突胶质细胞糖蛋白33-55(MOG33-55)配以完全弗氏佐剂(CFA)免疫小鼠,诱发EAE模型。通过神经功能评分、免疫组化染色以及酶联免疫吸附实验(ELISA),观察GBE对EAE小鼠的影响。结果 GBE组小鼠发病潜伏期[(12.5±0.64)d]较EAE组[(10.7±0.47)d]显著延长(P<0.05),并且GBE组发病高峰期(CFA免疫后第21天)神经功能评分较EAE组显著降低(P<0.05)。海马伞矢状切片免疫组化染色结果证实GBE组CD4+T淋巴细胞较EAE组明显减少,仅可见CD4+T淋巴细胞散在分布。ELISA检测结果显示发病高峰期GBE组TNF-α含量较EAE组明显降低(P<0.05)。结论 GBE可能通过抑制CD4+T淋巴细胞激活、减少炎症介质释放,从而保护延缓EAE小鼠脱髓鞘病变进程。Objective To explore the effect of Ginkgo biloba extract（GBE） on inflammatory demyelination of axons in mice with experimental autoimmune encephalomyelitis（EAE）. Methods Mice were divided into EAE model group（daily intraperitoneal injection of saline） and GBE treatment group（daily intraperitoneal injection of 70 mg/kg GBE）.Then all mice were given a subcutaneous injection of myelin oligodendrocyte glycoprotein 35-55（MOG33-55） emulsified in complete Freund＇s adjuvant（CFA） to induce EAE.The clinical scores,immunohistochemistry staining and ELISA were used to determine the effects of GBE in EAE mouse model. Results The onset latency of disease in GBE group was significantly delayed compared to EAE group[（12.5±0.64）d vs（10.7±0.47）d,P0.05].Furthermore,the mean clinical scores in GBE group showed a significant reduction at the peak of the disease compared with EAE group（P0.05）.The number of CD4＋ T cells was decreased after pretreatment with GBE and the fimbria had only few scattered CD4＋ T cells.The low levels of TNF-α were observed in GBE group at the peak of the disease. Conclusion GBE can delay demyelination process in EAE mice through an inhibition of cytokine release by activated CD4＋T cells,suggesting that GBE has potential to treat multiple sclerosis in future.

关 键 词：银杏提取物 自身免疫性脑脊髓炎 多发性硬化 T淋巴细胞 小鼠 

分 类 号：R363[医药卫生—病理学]