EGF-PE4O重组毒素对喉鳞癌细胞株Hep-2的毒性作用  

Cytotoxity of EGF-PE4O recombinant toxin on Hep-2 cell line of laryngeal squamous cell cancer

作　　者：任晓燕[1] 孟锐奇[2] 张欣欣[1] 朱冬冬 朱平[2] 董震[1] 杨占泉[1] 

机构地区：[1]白求恩医科大学第三临床医院耳鼻咽喉-头颈外科,长春130031 [2]解放军农牧大学军事兽医研究所

出　　处：《耳鼻咽喉（头颈外科）》1999年第6期356-359,共4页Chinese Arch Otolaryngology-Head Neck Surg

摘　　要：观察EGF－PE40重组毒素对喉鳞癌细胞的毒性作用，为寻找新型的治疗头颈鳞癌的生物制剂奠定基础。应用MTT比色法测定EGF－PE40重组毒素的生物学活性。并在光镜下观察Hep－2细胞形态变化。确定MTT比色法中Hep－2细胞浓度为2×104／well。选择10％SDS－0．01mol／LHCI作为MTT结晶的溶解液，当EGF－PE40重组毒素为0．53ng／ml时，50％的Hep－2细胞死亡，在一定范围内，随重组毒素增加，细胞损伤加重。Inorder to find a new kind of biological agent for the treatment of headand neck aquamous cell cancer,cytotoxic activity of recombinant toxin EGF-PE40(epi-dermal growth factor-pseudomonas exotoxins-40) on laryngeal squamous cancer cellswere observed. The biologic activity of EGF-PE40 recombinant toxin was measured byMTT colorimeric assay and Hep-2 cells were observed under light microscope. This as-say determined the concentration of Hep-2 cells to be 2 × 1O4 cells/well, selected lO%SDS-0. 01m0l/L HCL as s0lubilizers of MTT formazan. Cytotoxcity reached 50% withEGF-PE40 recombinant toxin at 0. 53ng/ml. Cell damage became more serious with re-combinant toxin increasing in a certain range.

关 键 词：细胞毒性 喉癌 鳞癌 EGF-PE40 重组毒素 

分 类 号：R739.650.5[医药卫生—肿瘤]