Extraction Optimization of Polysaccharides from Pitaya Stems  

作　　者：HE Cong-fen LI Peng ZHAO Hua SONG Li-ya ZHU Jun DONG Yin-mao 

机构地区：[1]Beijing Key Lab of Plant Resources Research and Development,Beijing Technology and Business University,Beijing 100048 [2]College of Life Science,Capital Normal University,Beijing 100037

出　　处：《Agricultural Science & Technology》2011年第7期947-949,979,共4页农业科学与技术（英文版）

摘　　要：[Objective]The aim was to describe the extraction of polysaccharides from pitaya stems.[Method]The hot water,enzyme-assisted and microwave-assisted methods were used,with the microwave-assisted extraction being deemed optimal by general evaluation.[Result]The main factors affecting the yield of polysaccharides in the microwave-assisted extraction,by order of magnitude,were as follows:time >microwave power >temperature;additionally,optimal conditions included a 10 min extraction time,an 80℃ extraction temperature and a microwave setting of 200 W.Using these optimal conditions,the yield of PSPS(Polysaccharides from Pitaya Stems) was 1.42%.After purification,the yield of PSPS was 0.74%.[Conclusion]The PSPS was analyzed by IR,MALDI-TOF-MS and an element analysis technique.It was shown to be a polysaccharide mixture,and the molecular weight was between 3 900 and 4 300 Da.[Objective] The aim was to describe the extraction of polysaccharides from pitaya stems.[Method] The hot water,enzyme-assisted and microwave-assisted methods were used,with the microwave-assisted extraction being deemed optimal by general evaluation.[Result] The main factors affecting the yield of polysaccharides in the microwave-assisted extraction,by order of magnitude,were as follows：timemicrowave powertemperature;additionally,optimal conditions included a 10 min extraction time,an 80 ℃ extraction temperature and a microwave setting of 200 W.Using these optimal conditions,the yield of PSPS（Polysaccharides from Pitaya Stems） was 1.42%.After purification,the yield of PSPS was 0.74%.[Conclusion] The PSPS was analyzed by IR,MALDI-TOF-MS and an element analysis technique.It was shown to be a polysaccharide mixture,and the molecular weight was between 3 900 and 4 300 Da.

关 键 词：Stem of pitaya POLYSACCHARIDE EXTRACTION Process optimization Structure identification 

分 类 号：TS201.25[轻工技术与工程—食品科学] S667.9[轻工技术与工程—食品科学与工程]